Zhu Chao, Wu Shengbing, Wu Xin, Zhou Meiqi, Wang Kun, Cui Shuai, Zhou Jie
Graduate School, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Evid Based Complement Alternat Med. 2022 Mar 21;2022:2114517. doi: 10.1155/2022/2114517. eCollection 2022.
Wushu acupoints are the five acupoints distributed below the human elbow and knee joint. They are all located on the same meridian and divided into five categories: Jing, Ying, Shu, Jing, and He. It has been shown that electroacupuncture (EA) at Shenmen point of heart meridian can improve acute myocardial ischemia (AMI) early. However, it is still unclear if all the Wushu acupoints of the heart meridian can improve AMI. Hence, this study emphasizes Wushu acupoints of heart meridian, compares them with Wushu acupoints of lung meridian, and studies the therapeutic effect of EA at Wushu acupoints on AMI and its possible mechanism. It also discusses the specificity of the heart meridian to heart disease. The AMI model is established by ligation of the left anterior descending coronary artery. The detection methods like the physiological recorder, TTC staining, ELISA, and so forth were used to determine the ECG, myocardial infarct size, serum myocardial enzymes, and myocardial tissue-related protein expression in rats. The heart rate (HR) and ST segment along with creatine kinase (CK), creatine kinase isoenzymes (CK-MB), lactate dehydrogenase (LDH), and myocardial infarctions increased after the induction with AMI. Furthermore, the expressions of PINK1 and Parkin protein also showed an increase. However, EA at Wushu acupoints in the heart meridian can reverse the above changes, whereas EA at the lung meridian exhibits limited effect. It is depicted that the heart meridian has a relatively specific relationship with the heart in a diseased state.
五输穴是分布于人体肘、膝关节以下的五个穴位。它们均位于同一条经络上，分为井、荥、输、经、合五类。研究表明，心经神门穴电针可早期改善急性心肌缺血（AMI）。然而，心经所有五输穴是否都能改善AMI仍不清楚。因此，本研究着重于心经五输穴，将其与肺经五输穴进行比较，研究心经五输穴电针对AMI的治疗作用及其可能机制。还探讨了心经对心脏病的特异性。通过结扎左冠状动脉前降支建立AMI模型。使用生理记录仪、TTC染色、ELISA等检测方法测定大鼠的心电图、心肌梗死面积、血清心肌酶及心肌组织相关蛋白表达。诱导AMI后，心率（HR）、ST段以及肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）和心肌梗死面积均增加。此外，PINK1和Parkin蛋白的表达也呈增加趋势。然而，心经五输穴电针可逆转上述变化，而肺经五输穴电针的作用有限。结果表明，在疾病状态下，心经与心脏具有相对特定的关系。
