• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过固定在具有优化表面结构的聚合物刷上来激活和稳定南极假丝酵母脂肪酶B

Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure.

作者信息

Wunschik Dennis Sebastian, Lorenz André, Ingenbosch Kim Nadine, Gutmann Jochen Stefan, Hoffmann-Jacobsen Kerstin

机构信息

Chemistry Department, Institute for Coatings and Surface Chemistry, Niederrhein University of Applied Sciences, Adlerstr. 32, 47798, Krefeld, Germany.

Deutsches Textilforschungszentrum Nord-West gGmbH, Adlerstr. 1, 47798, Krefeld, Germany.

出版信息

Appl Biochem Biotechnol. 2022 Aug;194(8):3384-3399. doi: 10.1007/s12010-022-03913-9. Epub 2022 Mar 31.

DOI:10.1007/s12010-022-03913-9
PMID:35357660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9270307/
Abstract

A reusable support system for the immobilization of lipases is developed using hybrid polymer-inorganic core shell nanoparticles. The biocatalyst core consists of a silica nanoparticle. PMMA is grafted from the nanoparticle as polymer brush via ARGET ATRP (activator regenerated by electron transfer atom transfer radical polymerization), which allows defining the surface properties by chemical synthesis conditions. Lipase B from Candida antarctica is immobilized on the hybrid particles. The activity and stability of the biocatalyst are analyzed by spectroscopic activity analysis. It is shown that the hydrophobic PMMA brushes provide an activating surface for the lipase giving a higher specific activity than the enzyme in solution. Varying the surface structure from disordered to ordered polymer brushes reveals that the reusability of the biocatalyst is more effectively optimized by the surface structure than by the introduction of crosslinking with glutaraldehyde (GDA). The developed immobilization system is highly suitable for biocatalysis in non-native media which is shown by a transesterification assay in isopropyl alcohol and an esterification reaction in n-heptane.

摘要

利用杂化聚合物-无机核壳纳米粒子开发了一种用于固定化脂肪酶的可重复使用的支撑系统。生物催化剂核心由二氧化硅纳米粒子组成。通过ARGET ATRP（电子转移原子转移自由基聚合再生活化剂）从纳米粒子接枝PMMA作为聚合物刷，这允许通过化学合成条件定义表面性质。将来自南极假丝酵母的脂肪酶B固定在杂化颗粒上。通过光谱活性分析来分析生物催化剂的活性和稳定性。结果表明，疏水性PMMA刷为脂肪酶提供了一个活化表面，其比溶液中的酶具有更高的比活性。将表面结构从无序聚合物刷变为有序聚合物刷表明，通过表面结构比通过引入戊二醛（GDA）交联能更有效地优化生物催化剂的可重复使用性。在异丙醇中的酯交换测定和在正庚烷中的酯化反应表明，所开发的固定化系统非常适合在非天然介质中进行生物催化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/6674b3a901b5/12010_2022_3913_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/12846b8d83cf/12010_2022_3913_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/0ae813b8025c/12010_2022_3913_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/bfc6fc73d302/12010_2022_3913_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/b69d994d5d53/12010_2022_3913_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/a2681ad78631/12010_2022_3913_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/52fec3965bae/12010_2022_3913_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/e82ab491597c/12010_2022_3913_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/6674b3a901b5/12010_2022_3913_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/12846b8d83cf/12010_2022_3913_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/0ae813b8025c/12010_2022_3913_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/bfc6fc73d302/12010_2022_3913_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/b69d994d5d53/12010_2022_3913_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/a2681ad78631/12010_2022_3913_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/52fec3965bae/12010_2022_3913_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/e82ab491597c/12010_2022_3913_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6817/9270307/6674b3a901b5/12010_2022_3913_Fig8_HTML.jpg

相似文献

1
Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure.通过固定在具有优化表面结构的聚合物刷上来激活和稳定南极假丝酵母脂肪酶B
Appl Biochem Biotechnol. 2022 Aug;194(8):3384-3399. doi: 10.1007/s12010-022-03913-9. Epub 2022 Mar 31.
2
Enhancement of immobilized lipase activity by design of polymer brushes on a hollow fiber membrane.通过在中空纤维膜上设计聚合物刷来提高固定化脂肪酶的活性。
J Biosci Bioeng. 2015 Sep;120(3):257-62. doi: 10.1016/j.jbiosc.2015.01.009. Epub 2015 Feb 19.
3
Erratum: Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification.勘误：用于蛋白质纯化的聚（丙烯酸五氟苯酯）功能化二氧化硅微珠的制备
J Vis Exp. 2019 Apr 30(146). doi: 10.3791/6328.
4
Understanding Design Rules for Optimizing the Interface between Immobilized Enzymes and Random Copolymer Brushes.理解优化固定化酶与无规共聚物刷之间界面的设计规则。
ACS Appl Mater Interfaces. 2021 Jun 16;13(23):26694-26703. doi: 10.1021/acsami.1c02443. Epub 2021 Jun 3.
5
Influence of the morphology of core-shell supports on the immobilization of lipase B from Candida antarctica.核壳载体形态对南极假丝酵母脂肪酶B固定化的影响。
Molecules. 2014 Aug 18;19(8):12509-30. doi: 10.3390/molecules190812509.
6
Immobilization of Candida rugosa lipase for resolution of racimic ibuprofen.固定化假丝酵母脂肪酶拆分消旋布洛芬。
Daru. 2021 Jun;29(1):117-123. doi: 10.1007/s40199-021-00388-7. Epub 2021 Feb 2.
7
Electrospun regenerated cellulose nanofibrous membranes surface-grafted with polymer chains/brushes via the atom transfer radical polymerization method for catalase immobilization.通过原子转移自由基聚合方法，在静电纺再生纤维素纳米纤维膜表面接枝聚合物链/刷，用于固定化过氧化氢酶。
ACS Appl Mater Interfaces. 2014 Dec 10;6(23):20958-67. doi: 10.1021/am505722g. Epub 2014 Nov 21.
8
Kinetic study of Candida antarctica lipase B immobilization using poly(methyl methacrylate) nanoparticles obtained by miniemulsion polymerization as support.以通过细乳液聚合制备的聚甲基丙烯酸甲酯纳米粒子为载体固定化南极假丝酵母脂肪酶B的动力学研究
Appl Biochem Biotechnol. 2015 Mar;175(6):2961-71. doi: 10.1007/s12010-015-1478-5. Epub 2015 Jan 13.
9
Immobilization of Candida antarctica Lipase on Nanomaterials and Investigation of the Enzyme Activity and Enantioselectivity.固定化南极假丝酵母脂肪酶于纳米材料及对酶活性和对映选择性的研究。
Appl Biochem Biotechnol. 2021 Feb;193(2):430-445. doi: 10.1007/s12010-020-03443-2. Epub 2020 Oct 6.
10
Comparative Study of Polymer-Grafted BaTiO Nanoparticles Synthesized Using Normal ATRP as Well as ATRP and ARGET-ATRP with Sacrificial Initiator with a Focus on Controlling the Polymer Graft Density and Molecular Weight.采用常规原子转移自由基聚合（ATRP）以及带有牺牲引发剂的 ATRP 和ARGET-ATRP 对比研究接枝聚合物的 BaTiO3 纳米粒子，重点在于控制聚合物接枝密度和分子量。
Molecules. 2023 May 30;28(11):4444. doi: 10.3390/molecules28114444.

引用本文的文献

1
Self-Assembled Immobilization and Metal-Polyphenol Network Encapsulation of β-Galactosidase on T4 Phage for Enhanced Biocatalytic Performance.β-半乳糖苷酶在T4噬菌体上的自组装固定化及金属-多酚网络封装以增强生物催化性能
Appl Biochem Biotechnol. 2025 May 14. doi: 10.1007/s12010-025-05267-4.
2
Design of a Biocatalytic Filter for the Degradation of Diclofenac and Its Ozonation Products.用于降解双氯芬酸及其臭氧化产物的生物催化过滤器的设计
Eng Life Sci. 2025 May 2;25(5):e70024. doi: 10.1002/elsc.70024. eCollection 2025 May.
3
Lipase Activation by Poly(Methyl Methacrylate) in Dispersed Solution: Mechanistic Insights by Fluorescence Spectroscopy.

本文引用的文献

1
Cosolvency-Induced Switching of the Adhesion between Poly(methyl methacrylate) Brushes.共溶剂诱导的聚甲基丙烯酸甲酯刷间粘附力的转变
ACS Macro Lett. 2015 Jan 20;4(1):75-79. doi: 10.1021/mz500775w. Epub 2014 Dec 26.
2
A fluorescence-based activity assay for immobilized lipases in non-native media.一种用于非天然介质中固定化脂肪酶的基于荧光的活性测定法。
Anal Biochem. 2019 Mar 15;569:22-27. doi: 10.1016/j.ab.2019.01.005. Epub 2019 Jan 17.
3
Poly(carboxybetaine methacrylate)-functionalized magnetic composite particles: A biofriendly support for lipase immobilization.
聚甲基丙烯酸甲酯在分散溶液中对脂肪酶的激活作用：通过荧光光谱法的机理洞察
Appl Biochem Biotechnol. 2025 Mar 31. doi: 10.1007/s12010-025-05217-0.
4
Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes.各种酶固定化技术和载体的优缺点。
Appl Biochem Biotechnol. 2024 Sep;196(9):5633-5655. doi: 10.1007/s12010-023-04838-7. Epub 2024 Jan 4.
5
Glutaraldehyde-crosslinked Rhizopus oryzae whole cells show improved catalytic performance in alkene epoxidation.戊二醛交联米根霉完整细胞在烯烃环氧化反应中表现出更好的催化性能。
Microb Cell Fact. 2023 Feb 22;22(1):33. doi: 10.1186/s12934-023-02026-0.
聚（羧酸甜菜碱甲基丙烯酸酯）功能化磁性复合粒子：一种用于脂肪酶固定化的生物友好型载体。
Int J Biol Macromol. 2018 Feb;107(Pt B):2660-2666. doi: 10.1016/j.ijbiomac.2017.10.150. Epub 2017 Oct 26.
4
Kinetics of Detergent-Induced Activation and Inhibition of a Minimal Lipase.去污剂诱导的最小脂肪酶激活与抑制的动力学
J Phys Chem B. 2017 Feb 16;121(6):1248-1257. doi: 10.1021/acs.jpcb.6b11037. Epub 2017 Feb 6.
5
Controlled and tunable design of polymer interface for immobilization of enzymes: does curvature matter?聚合物界面的可控和可调设计用于固定化酶：曲率重要吗？
Soft Matter. 2017 Feb 7;13(5):1074-1084. doi: 10.1039/c6sm02380k. Epub 2017 Jan 17.
6
Lipase catalysis in organic solvents: advantages and applications.有机溶剂中的脂肪酶催化作用：优势与应用
Biol Proced Online. 2016 Jan 13;18:2. doi: 10.1186/s12575-016-0033-2. eCollection 2016.
7
Interfacial Activation of Candida antarctica Lipase B: Combined Evidence from Experiment and Simulation.南极假丝酵母脂肪酶B的界面激活：实验与模拟的综合证据
Biochemistry. 2015 Sep 29;54(38):5969-79. doi: 10.1021/acs.biochem.5b00586. Epub 2015 Sep 15.
8
Immobilization of lipases on hydrophobic supports involves the open form of the enzyme.将脂肪酶固定在疏水载体上涉及到酶的开放形式。
Enzyme Microb Technol. 2015 Apr;71:53-7. doi: 10.1016/j.enzmictec.2015.02.001. Epub 2015 Feb 9.
9
Industrial applications of enzyme biocatalysis: Current status and future aspects.酶生物催化的工业应用：现状与未来展望。
Biotechnol Adv. 2015 Nov 15;33(7):1443-54. doi: 10.1016/j.biotechadv.2015.02.014. Epub 2015 Mar 6.
10
Enzyme immobilisation in biocatalysis: why, what and how.酶在生物催化中的固定化：原因、方法和应用。
Chem Soc Rev. 2013 Aug 7;42(15):6223-35. doi: 10.1039/c3cs60075k.