Department of Biochemistry, Biotechnical Faculty, University of Bihać, Luke Marjanovića bb, 77000, Bihać, Bosnia and Herzegovina.
Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000, Split, Croatia.
Chem Biodivers. 2022 May;19(5):e202101002. doi: 10.1002/cbdv.202101002. Epub 2022 Apr 12.
Clinopidium vulgare L. (wild basil, Lamiaceae) is a well-known medicinal plant used in the traditional medicine in many countries. Medicinal plants present potential sources of bioactive compounds. Many of them are rich in polyphenol compounds that show biological potential in terms of protecting biological molecules from oxidation and in inhibition of cholinesterase enzymes, which may be significant in the treatment of diseases related to oxidative stress. In this work, we examined the chemical composition of Clinopodium vulgare L. hot water and methanol extract using spectroscopic and HPLC/DAD techniques. Using DPPH and FRAP methods the antioxidant activity was analyzed. The ability to protect proteins and lipids from oxidation was also determined as well as the ability of extracts to inhibit cholinesterase enzymes using Ellman's method. Analyzed extracts were rich in polyphenol compounds. Among 16 identified and quantified phenolic compounds dominant were: rosmarinic (26.63 and 34.21 mg/g) and ellagic acid (23.11 and 29.31 mg/g) of hot water and methanol extract, respectively. They show good antioxidant activity and good potential in protecting lipids from oxidation. The ability of extracts to inhibit enzyme acetylcholinesterase was weak, while inhibition of the butyrylcholinesterase was missing. Extracts show prooxidant activity in terms of protecting proteins from oxidation.
普通筋骨草（筋骨草，唇形科）是一种在许多国家的传统医学中被广泛应用的药用植物。药用植物是生物活性化合物的潜在来源。其中许多植物富含多酚化合物，这些化合物在保护生物分子免受氧化和抑制胆碱酯酶方面具有生物潜力，这在治疗与氧化应激相关的疾病方面可能具有重要意义。在这项工作中，我们使用光谱和 HPLC/DAD 技术研究了筋骨草热水和甲醇提取物的化学成分。采用 DPPH 和 FRAP 方法分析了抗氧化活性。还测定了提取物保护蛋白质和脂质免受氧化的能力，以及用 Ellman 法测定提取物抑制胆碱酯酶的能力。分析的提取物富含多酚化合物。在所鉴定和定量的 16 种酚类化合物中，热水和甲醇提取物中分别以迷迭香酸（26.63 和 34.21mg/g）和鞣花酸（23.11 和 29.31mg/g）为主。它们具有良好的抗氧化活性和保护脂质免受氧化的潜力。提取物抑制乙酰胆碱酯酶的能力较弱，而对丁酰胆碱酯酶的抑制作用则缺失。从保护蛋白质免受氧化的角度来看，提取物具有促氧化活性。
