Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Department of Biology, Tufts University, Medford, MA, USA.
Methods Mol Biol. 2022;2450:51-67. doi: 10.1007/978-1-0716-2172-1_3.
Physarum polycephalum is a protist slime mould that exhibits a high degree of responsiveness to its environment through a complex network of tubes and cytoskeletal components that coordinate behavior across its unicellular, multinucleated body. Physarum has been used to study decision making, problem solving, and mechanosensation in aneural biological systems. The robust generative and repair capacities of Physarum also enable the study of whole-body regeneration within a relatively simple model system. Here we describe methods for growing, imaging, quantifying, and sampling Physarum that are adapted for investigating regeneration and repair.
多头绒泡菌是一种原生质黏菌，通过复杂的管网和细胞骨架组件网络对环境表现出高度的响应性，从而协调其单细胞、多核体的行为。多头绒泡菌已被用于研究无神经生物系统中的决策制定、问题解决和机械感觉。多头绒泡菌强大的生成和修复能力还使人们能够在相对简单的模型系统中研究全身再生。在这里，我们描述了适应于研究再生和修复的多头绒泡菌的培养、成像、定量和采样方法。
