Mi Jinglin, Ma Shanshan, Chen Wei, Kang Min, Xu Meng, Liu Chang, Li Bo, Wu Fang, Liu Fengju, Zhang Yong, Wang Rensheng, Jiang Li
Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Department of Oncology, Yunfu People's Hospital, Yunfu, China.
Front Oncol. 2022 Mar 11;12:772816. doi: 10.3389/fonc.2022.772816. eCollection 2022.
KIF15 plays a vital role in many biological processes and has been reported to influence the occurrence and development of certain human cancers. However, there are few systematic evaluations on the role of KIF15 in human cancers, and the role of KIF15 in the diagnosis and prognosis of nasopharyngeal carcinoma (NPC) also remains unexplored. Therefore, this study aimed to conduct a pan-cancer analysis of KIF15 and evaluate its diagnostic and prognostic potential in NPC.
The expression pattern, prognostic value, molecular function, tumor mutation burden, microsatellite instability, and immune cell infiltration of KIF15 were examined based on public databases. Next, the diagnostic value of KIF15 in NPC was analyzed using the Gene Expression Omnibus (GEO) database and immunohistochemistry (IHC). Kaplan-Meier curves, Cox regression analyses, and nomograms were used to evaluate the effects of KIF15 expression on NPC prognosis. Finally, the effect of KIF15 on NPC was explored by experiments.
The expression of KIF15 was significantly upregulated in 20 out of 33 cancer types compared to adjacent normal tissue. Kyoto Encyclopedia of Genes and Genomes enrichment (KEGG) analysis showed that KIF15 could participate in several cancer-related pathways. The increased expression level of KIF15 was correlated with worse clinical outcomes in many types of human cancers. Additionally, KIF15 expression was related to cancer infiltration of immune cells, tumor mutation burden, and microsatellite instability. In the analysis of NPC, KIF15 was significantly upregulated based on the GEO database and immunohistochemistry. A high expression of KIF15 was negatively associated with the prognosis of patients with NPC. A nomogram model integrating clinical characteristics and KIF15 expression was established, and it showed good predictive ability with an area under the curve value of 0.73. KIF15 knockdown significantly inhibited NPC cell proliferation and migration.
Our findings revealed the important and functional role of KIF15 as an oncogene in pan-cancer. Moreover, high expression of KIF15 was found in NPC tissues, and was correlated with poor prognosis in NPC. KIF15 may serve as a potential therapeutic target in NPC treatment.
KIF15在许多生物学过程中发挥着至关重要的作用，据报道它会影响某些人类癌症的发生和发展。然而，关于KIF15在人类癌症中的作用，目前缺乏系统的评估，其在鼻咽癌（NPC）诊断和预后中的作用也尚未得到探索。因此，本研究旨在对KIF15进行泛癌分析，并评估其在NPC中的诊断和预后潜力。
基于公共数据库检测KIF15的表达模式、预后价值、分子功能、肿瘤突变负担、微卫星不稳定性和免疫细胞浸润情况。接下来，利用基因表达综合数据库（GEO）和免疫组织化学（IHC）分析KIF15在NPC中的诊断价值。采用Kaplan-Meier曲线、Cox回归分析和列线图评估KIF15表达对NPC预后的影响。最后，通过实验探索KIF15对NPC的作用。
与相邻正常组织相比，KIF15在33种癌症类型中的20种中表达显著上调。京都基因与基因组百科全书富集（KEGG）分析表明，KIF15可参与多种癌症相关途径。KIF15表达水平的升高与多种类型人类癌症的较差临床结局相关。此外，KIF15表达与免疫细胞的癌症浸润、肿瘤突变负担和微卫星不稳定性有关。在NPC分析中，基于GEO数据库和免疫组织化学，KIF15显著上调。KIF15的高表达与NPC患者的预后呈负相关。建立了一个整合临床特征和KIF15表达的列线图模型，其曲线下面积值为0.73，显示出良好的预测能力。KIF敲低显著抑制NPC细胞增殖和迁移。
我们的研究结果揭示了KIF15作为一种癌基因在泛癌中的重要功能作用。此外，在NPC组织中发现KIF15高表达，且与NPC的不良预后相关。KIF15可能作为NPC治疗的潜在靶点。
