do Vale E, Gerstmeier H, Meurer M, Krieg T, Braun-Falco O
Dtsch Med Wochenschr. 1986 Dec 12;111(50):1922-7. doi: 10.1055/s-2008-1068736.
Sera from 56 patients with circumscribed scleroderma (CS) were tested for the presence of antinuclear antibodies (ANA) by indirect immunofluorescence on HEp-2-cells. 16 patients (28.6%) had ANA, mostly in low titers of 1: 40 and with uncharacteristic fluorescence patterns. Nucleolar antibodies as in progressive systemic sclerosis were seen in a few cases only. Two female patients demonstrated antibodies with a centromere-like pattern. ANA were most frequently found in patients with atrophodermia idiopathica progressiva (60%) and in linear CS (44%). Rheumatoid factor was present in 62% of patients with linear CS; it was less frequent in other forms of CS. Antibodies against double-stranded DNA were found only in one female patient, but three patients with systemic involvement had antibodies to single-stranded DNA. Antibodies to soluble nuclear Scl-70, Ul-RNP and LA(SSB) antigens or to the cytoplasmatic Ro(SSA)-antigen, respectively, were not detected in the patients with CS. These results demonstrate that humoral autoimmune phenomena may occur in CS, mainly in patients with linear CS. These phenomena, however, are less frequent and specific than in progressive systemic sclerosis or in other collagen vascular diseases.
采用间接免疫荧光法，以人喉癌上皮细胞（HEp-2细胞）检测了56例局限性硬皮病（CS）患者血清中的抗核抗体（ANA）。16例患者（28.6%）存在ANA，大多为低滴度（1:40），荧光模式无特征性。仅少数病例出现了进行性系统性硬化症中的核仁抗体。两名女性患者出现了着丝粒样模式的抗体。ANA最常见于进行性特发性皮肤萎缩患者（60%）和线状CS患者（44%）。类风湿因子见于62%的线状CS患者；在其他形式的CS中较少见。仅一名女性患者发现了抗双链DNA抗体，但三名有系统性受累的患者存在抗单链DNA抗体。CS患者未检测到针对可溶性核Scl-70、U1-RNP和La（SSB）抗原或细胞质Ro（SSA）抗原的抗体。这些结果表明，体液自身免疫现象可能发生在CS中，主要见于线状CS患者。然而，这些现象比进行性系统性硬化症或其他胶原血管疾病中出现的频率更低且特异性更差。
