Department of Animal Breeding, INIA-CSIC, Madrid, Spain.
Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Reprod Fertil Dev. 2022 Jun;34(10):699-712. doi: 10.1071/RD21209.
The present work aimed to evaluate the chromatin compaction of rooster spermatozoa along the male reproductive tract, and to study the vas deferens lining cells, potentially involved in sperm maturation. Chromomycin A3 (CMA3) was used to determine the chromatin compaction of spermatozoa from testis (T), proximal (including epididymis, V1), intermediate (V2) and distal (V3) vas deferens, and ejaculate (E). Six Birchen Leonesa roosters were used. E was obtained in vivo by dorso-ventral massage. V1, V2 and V3 sperm were obtained post mortem (six pairs of vasa deferentia), by flushing. T was obtained by washing the testes, cut in halves. The fixed cells were stained with CMA3 and propidium iodide for flow cytometry assessment. Results showed higher (P P P.
本研究旨在评估公鸡精子在雄性生殖道中的染色质紧缩情况，并研究可能参与精子成熟的输精管衬里细胞。使用 Chromomycin A3（CMA3）来确定来自睾丸（T）、近端（包括附睾、V1）、中间（V2）和远端（V3）输精管以及精液（E）的精子的染色质紧缩情况。使用了六只比森霍勒纳罗鸡。通过背-腹按摩在体内获得 E。死后通过冲洗获得 V1、V2 和 V3 精子（六对输精管）。通过洗涤睾丸获得 T，将其切成两半。固定细胞用 CMA3 和碘化丙啶染色，用于流式细胞术评估。结果表明，近端和远端输精管中的精子染色质紧缩程度高于睾丸和精液中的精子（P < 0.05）。
