Pinsard Marion, Mouchet Nicolas, Dion Ludivine, Bessede Thomas, Bertrand Martin, Darai Emile, Bellaud Pascale, Loget Philippe, Mazaud-Guittot Séverine, Morandi Xavier, Leveque Jean, Lavoué Vincent, Duraes Martha, Nyangoh Timoh Krystel
Department of Obstetrics and Gynecology, Hopital Universitaire de Rennes, Rennes, France.
University Rennes 1, CNRS, Inserm UMS Biosit, France BioImaging, Core Facility H2P2 Rennes, France.
Fertil Steril. 2022 Jun;117(6):1279-1288. doi: 10.1016/j.fertnstert.2022.02.013. Epub 2022 Mar 30.
To better understand the physiology of pain in pelvic pain pathological conditions, such as endometriosis, in which alterations of uterine innervation have been highlighted, we performed an anatomic and functional mapping of the macro- and microinnervation of the human uterus. Our aim was to provide a 3-dimensional reconstruction model of uterine innervation.
This was an experimental study. We dissected the pelvises of 4 human female fetuses into serial sections, and treated them with hematoxylin and eosin staining before immunostaining.
Academic Research Unit.
None.
None.
Detection of nerves (S100 +) and characterization of the types of nerves. The slices obtained were aligned to construct a 3-dimensional model.
A 3-dimensional model of uterine innervation was constructed. The nerve fibers appeared to have a centripetal path from the uterine serosa to the endometrium. Within the myometrium, innervation was dense. Endometrial innervation was sparse but present in the functional layer of the endometrium. Overall innervation was richest in the supravaginal cervix and rarer in the body of the uterus. Innervation was rich particularly laterally to the cervix next to the parametrium and paracervix. Four types of nerve fibers were identified: autonomic sympathetic (TH+), parasympathetic (VIP+), and sensitive (NPY+, CGRP1+ and VIP+). They were found in the 3 portions and the 3 layers of the uterus.
We constructed a 3-dimensional model of the human uterine innervation. This model could provide a solid base for studying uterine innervation in pathologic situations, in order to find new therapeutic approaches.
为了更好地理解盆腔疼痛病理状况（如子宫内膜异位症，其中子宫神经支配的改变已得到突出显示）下的疼痛生理学，我们对人类子宫的宏观和微观神经支配进行了解剖学和功能绘图。我们的目的是提供子宫神经支配的三维重建模型。
这是一项实验研究。我们将4例人类女性胎儿的骨盆解剖成连续切片，并在免疫染色前用苏木精和伊红染色处理。
学术研究单位。
无。
无。
检测神经（S100 +）并对神经类型进行特征描述。将获得的切片对齐以构建三维模型。
构建了子宫神经支配的三维模型。神经纤维似乎有一条从子宫浆膜到子宫内膜的向心路径。在子宫肌层内，神经支配密集。子宫内膜神经支配稀疏，但存在于子宫内膜的功能层。总体神经支配在阴道上宫颈最丰富，在子宫体较少见。神经支配在宫颈旁侧尤其是靠近子宫旁组织和子宫颈旁组织处丰富。鉴定出四种神经纤维类型：自主交感神经（TH +）、副交感神经（VIP +）和感觉神经（NPY +、CGRP1 +和VIP +）。它们存在于子宫的三个部分和三层中。
我们构建了人类子宫神经支配的三维模型。该模型可为研究病理情况下的子宫神经支配提供坚实基础，以便找到新的治疗方法。
