Tokushige Natsuko, Markham Robert, Russell Peter, Fraser Ian S
Department of Obstetrics and Gynaecology, Queen Elizabeth II Research Institute for Mothers and Infants, University of Sydney, Sydney, Australia.
Fertil Steril. 2007 Oct;88(4):795-803. doi: 10.1016/j.fertnstert.2006.12.078. Epub 2007 Apr 23.
To investigate types of nerve fibers in endometrium and myometrium in women with endometriosis.
Laboratory study using human tissue.
University-based laboratory.
PATIENT(S): Women with and without endometriosis undergoing hysterectomy.
INTERVENTION(S): Histologic sections of contiguous endometrial and myometrial tissues were prepared from hysterectomies performed on women with and without endometriosis.
MAIN OUTCOME MEASURE(S): Types and density of nerve fibers in endometrium and myometrium in women with and without endometriosis were determined using a series of specific markers for neuronal structure and function: PGP9.5, NF, SP, CGRP, TH, VAChT, VIP, and NPY.
RESULT(S): Nerve fibers stained with PGP9.5 and NF in endometrium and myometrium were significantly increased in women with endometriosis compared with women without endometriosis. Nerve fibers in the functional layer of endometrium in women with endometriosis were likely to be sensory C, a mixture of sensory A delta, sensory C, and adrenergic fibers in the basal layer of the endometrium, a mixture of sensory A delta, sensory C, adrenergic and cholinergic fibers in the myometrium.
CONCLUSION(S): Increased nerve fiber density in endometrium and myometrium, and sensory C fibers and adrenergic nerve fibers in the endometrium in women with endometriosis may play an important role in the mechanisms of pain generation in this condition.
研究子宫内膜异位症患者子宫内膜和肌层中神经纤维的类型。
使用人体组织的实验室研究。
大学实验室。
接受子宫切除术的有和没有子宫内膜异位症的女性。
从有和没有子宫内膜异位症的女性子宫切除术中获取连续的子宫内膜和肌层组织切片。
使用一系列针对神经元结构和功能的特异性标志物（PGP9.5、NF、SP、CGRP、TH、VAChT、VIP和NPY）来确定有和没有子宫内膜异位症的女性子宫内膜和肌层中神经纤维的类型和密度。
与没有子宫内膜异位症的女性相比，子宫内膜异位症女性的子宫内膜和肌层中PGP9.5和NF染色的神经纤维显著增加。子宫内膜异位症女性子宫内膜功能层的神经纤维可能是感觉C纤维，子宫内膜基底层是感觉Aδ、感觉C和肾上腺素能纤维的混合物，肌层是感觉Aδ、感觉C、肾上腺素能和胆碱能纤维的混合物。
子宫内膜异位症女性子宫内膜和肌层中神经纤维密度增加，以及子宫内膜中的感觉C纤维和肾上腺素能神经纤维可能在这种疾病的疼痛产生机制中起重要作用。
