Marinsek Gabriela Pustiglione, Choueri Paloma Kachel Gusso, Choueri Rodrigo Brasil, de Souza Abessa Denis Moledo, Gonçalves Alexandre Rodrigo Nascimento, Bortolotto Lorihany Bogo, de Britto Mari Renata
São Paulo State University (Unesp), Coastal Campus, Department of Biological and Environmental Sciences, Animal Morphophysiology Laboratory, São Vicente, Brazil; São Paulo State University (Unesp), Institute for Advanced Studies of Ocean, São Vicente, Brazil.
São Paulo State University (Unesp), Coastal Campus, Department of Biological and Environmental Sciences, Research Group on Pollution and Aquatic Ecotoxicology, São Vicente, Brazil; Ecotoxicology Laboratory - Unisanta, Universidade Santa Cecília, R. Oswaldo Cruz, 277 - CP 11045-907 - Boqueirão, Santos - SP, Brazil.
Mar Pollut Bull. 2022 May;178:113590. doi: 10.1016/j.marpolbul.2022.113590. Epub 2022 Mar 31.
The gastrointestinal tract and its enteric nervous system are the first routes of food and xenobiotics uptake. Considering the importance of this organ, this study evaluated intestinal biomarkers of Sphoeroides testudineus integrating the data to generate tools for pollution assessment. The fish were collected in three sites of São Paulo Coast and their intestines were analyzed for biochemical, histology, and neuronal density and morphometry biomarkers. To evaluate the differences among the data, a PERMANOVA was applied, followed by a FA/PCA. The PERMANOVA indicated differences (P < 0.001) between the regions (RA, A1, and A2). Four factors were extracted from the FA/PCA (62% cumulative), showing that the animals from A2 presented severe alterations, mainly in intestinal morphometry and neuronal density. A1 alterations refer mainly to the increase of neuronal metabolism. Our results also evidence a gradient of environmental quality related to the protection level (AR > A1 > A2).
胃肠道及其肠神经系统是食物和外源性物质摄取的首要途径。鉴于该器官的重要性，本研究评估了黄鳍鲳的肠道生物标志物，并整合数据以生成污染评估工具。在圣保罗海岸的三个地点采集了鱼类样本，并对其肠道进行了生化、组织学、神经元密度和形态计量学生物标志物分析。为评估数据之间的差异，应用了PERMANOVA，随后进行了因子分析/主成分分析（FA/PCA）。PERMANOVA表明各区域（RA、A1和A2）之间存在差异（P < 0.001）。从FA/PCA中提取了四个因子（累积贡献率62%），表明来自A2的动物出现了严重变化，主要体现在肠道形态计量学和神经元密度方面。A1的变化主要指神经元代谢增加。我们的结果还证明了与保护水平相关的环境质量梯度（AR > A1 > A2）。
