Yang Caixia, Yang Jingyan, Tan Li, Tang Pan, Pen Ting, Gao Tinghui, Liu Sijing, Guo Jinlin
Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Evid Based Complement Alternat Med. 2022 Mar 24;2022:3784580. doi: 10.1155/2022/3784580. eCollection 2022.
Long-term body fatigue poses a threat to human health. To explore novel sources of antifatigue medicine and food, we developed a novel formula composed of wolfberry, figs, white lentils, raspberries, and maca (WFWRM) according to the theory of traditional Chinese medicine. In this study, we explored whether the administration of the WFWRM relieves fatigue. Thirty male Kunming mice were divided into three groups, which received either intragastric administration of saline, vitamin C (100 mg/kg), or WFWRM (1.00 g/kg) every day. After 30 days of treatment, all mice exhaustively performed weight-bearing swimming. Another ten mice that did not perform swimming were treated with saline for 30 days and used as sedentary control. The antifatigue effect and biochemical oxidation phenomena were assessed in the exercise-exhausted model and sedentary controls. The histopathological changes in the liver and kidney tissues of mice were observed by performing hematoxylin-eosin (HE) staining. After 30 days of oral administration, the liver and kidney tissues of mice were healthy and show no pathological changes. Compared to the fatigue model group, WFWRM significantly increased the rota-rod time of the mice. Also, the concentrations of lactic acid (LA), blood urea nitrogen (BUN), creatine kinase (CK), and lactate dehydrogenase (LDH) in the WFWRM group significantly reduced. On the contrary, the levels of hepatic glycogen (LG), muscle glycogen (MG), and serum glucose (GLU) increased in the WFWRM group. Besides, WFWRM markedly reduced the levels of malondialdehyde (MDA) but increased the levels of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD). Pearson correlation analysis indicated that the concentrations of the sources of energy (LG, MG, and GLU) significantly correlated with those of metabolites (BLA, BUN, CK, and LDH) and antioxidant levels (SOD, GSH-PX, and MDA). Overall, our results suggested that the supplementation of WFWRM could improve exercise capacity and relieve fatigue probably by normalizing energy metabolism and attenuating oxidation.
长期身体疲劳对人类健康构成威胁。为探索抗疲劳医药和食品的新来源，我们依据中医理论研发了一种由枸杞、无花果、白扁豆、树莓和玛咖组成的新型配方（WFWRM）。在本研究中，我们探究了给予WFWRM是否能缓解疲劳。将30只雄性昆明小鼠分为三组，分别每天灌胃生理盐水、维生素C（100mg/kg）或WFWRM（1.00g/kg）。治疗30天后，所有小鼠进行负重游泳直至力竭。另外10只未进行游泳的小鼠用生理盐水处理30天作为安静对照组。在运动疲劳模型和安静对照组中评估抗疲劳效果及生化氧化现象。通过苏木精-伊红（HE）染色观察小鼠肝脏和肾脏组织的组织病理学变化。口服30天后，小鼠的肝脏和肾脏组织健康，未显示病理变化。与疲劳模型组相比，WFWRM显著延长了小鼠的转棒时间。此外，WFWRM组中乳酸（LA）、血尿素氮（BUN）、肌酸激酶（CK）和乳酸脱氢酶（LDH）的浓度显著降低。相反，WFWRM组中肝糖原（LG）、肌糖原（MG）和血清葡萄糖（GLU）的水平升高。此外，WFWRM显著降低了丙二醛（MDA）水平，但提高了谷胱甘肽过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）的水平。Pearson相关性分析表明，能量来源（LG、MG和GLU）的浓度与代谢产物（BLA、BUN、CK和LDH）及抗氧化水平（SOD、GSH-PX和MDA）的浓度显著相关。总体而言，我们的结果表明，补充WFWRM可能通过使能量代谢正常化和减轻氧化来提高运动能力并缓解疲劳。
