Utsumi R, Kawamukai M, Aiba H, Himeno M, Komano T
J Bacteriol. 1986 Dec;168(3):1408-14. doi: 10.1128/jb.168.3.1408-1414.1986.
Expression of the adenylate cyclase gene (cya) in synchronized Escherichia coli cells was investigated by using the cya-lacZ protein and operon fusion plasmids. The regulation of cya expression during the cell cycle is characterized as follows: cya is expressed during cell elongation; expression is repressed during cell division; regulation is exerted at the transcriptional level. To test cya expression during cell elongation, we constructed a plasmid (pLCR1) in which the lacUV5 promoter operator was fused to the structural gene of cya and investigated the effect of cya expression by isopropyl-beta-D-thiogalactopyranoside (IPTG) on the cell division of cells containing pLCR1. By the addition of IPTG, cell division was inhibited and filaments were formed. Such an inhibitory effect was antagonized by adding cyclic GMP to the culture medium and was not observed in the crp mutant.
通过使用cya-lacZ蛋白和操纵子融合质粒，研究了同步化大肠杆菌细胞中腺苷酸环化酶基因（cya）的表达。细胞周期中cya表达的调控特征如下：cya在细胞伸长期间表达；在细胞分裂期间表达受到抑制；调控作用于转录水平。为了测试细胞伸长期间的cya表达，我们构建了一个质粒（pLCR1），其中lacUV5启动子操纵子与cya的结构基因融合，并研究了异丙基-β-D-硫代半乳糖苷（IPTG）诱导的cya表达对含有pLCR1的细胞的细胞分裂的影响。通过添加IPTG，细胞分裂受到抑制并形成丝状。向培养基中添加环鸟苷酸可拮抗这种抑制作用，而在crp突变体中未观察到这种抑制作用。
