大肠杆菌recA缺失衍生物的便捷构建。
Convenient construction of recA deletion derivatives of Escherichia coli.
作者信息
Lorence M C, Rupert C S
出版信息
J Bacteriol. 1983 Oct;156(1):458-9. doi: 10.1128/jb.156.1.458-459.1983.
Abstract
Two Escherichia coli K-12 Hfr strains have been constructed which transfer a recA deletion, which is highly linked to a Tn10 insertion conferring tetracycline resistance, early during conjugation. These strains transfer the recA deletion in opposite directions with different origins of transfer, allowing for preservation of desirable recipient strain markers either clockwise or counterclockwise of recA.
摘要
构建了两株大肠杆菌K-12 Hfr菌株，它们在接合早期转移一个recA缺失片段，该片段与赋予四环素抗性的Tn10插入紧密连锁。这些菌株以不同的转移起始点沿相反方向转移recA缺失片段，从而能够在recA的顺时针或逆时针方向保留理想的受体菌株标记。
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