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Convenient construction of recA deletion derivatives of Escherichia coli.大肠杆菌recA缺失衍生物的便捷构建。
J Bacteriol. 1983 Oct;156(1):458-9. doi: 10.1128/jb.156.1.458-459.1983.
2
Transfer of the IncJ plasmid R391 to recombination deficient Escherichia coli K12: evidence that R391 behaves as a conjugal transposon.IncJ质粒R391向重组缺陷型大肠杆菌K12的转移：R391作为接合转座子的证据。
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Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains.构建一种有助于在大肠杆菌菌株之间转移recA突变的高频重组（Hfr）菌株。
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Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.大肠杆菌srl recA区域的物理图谱：Tn10产生的插入和缺失分析
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Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome.转座子Tn10在大肠杆菌K-12染色体的srl recA区域产生的缺失。
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大肠杆菌recA缺失衍生物的便捷构建。

Convenient construction of recA deletion derivatives of Escherichia coli.

作者信息

Lorence M C, Rupert C S

出版信息

J Bacteriol. 1983 Oct;156(1):458-9. doi: 10.1128/jb.156.1.458-459.1983.

DOI:10.1128/jb.156.1.458-459.1983
PMID:6311804
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215109/
Abstract

Two Escherichia coli K-12 Hfr strains have been constructed which transfer a recA deletion, which is highly linked to a Tn10 insertion conferring tetracycline resistance, early during conjugation. These strains transfer the recA deletion in opposite directions with different origins of transfer, allowing for preservation of desirable recipient strain markers either clockwise or counterclockwise of recA.

摘要

构建了两株大肠杆菌K-12 Hfr菌株，它们在接合早期转移一个recA缺失片段，该片段与赋予四环素抗性的Tn10插入紧密连锁。这些菌株以不同的转移起始点沿相反方向转移recA缺失片段，从而能够在recA的顺时针或逆时针方向保留理想的受体菌株标记。