大肠杆菌K-12 metJBLF基因簇的metJB组件的物理组织
Physical organization of the metJB component of the Escherichia coli K-12 metJBLF gene cluster.
作者信息
Liljestrand-Golden C A, Johnson J R
出版信息
J Bacteriol. 1984 Feb;157(2):413-9. doi: 10.1128/jb.157.2.413-419.1984.
Abstract
The structures of a series of plaque-forming metJB transducing phage were studied by restriction endonuclease mapping and enzyme activity assay. One of these phage, lambda pmet100, was inactivated by heat shock in the presence of EDTA, and deletion mutants were selected from the survivors. Two of these mutants, lambda pmet100 delta 1 and lambda pmet100 delta 2, were used to confirm the gene order metJ metB when moving clockwise on the linkage map of Escherichia coli K-12. Additional results indicate that the metB gene can be expressed independently of any other component of the met regulon and that the metJ gene also forms a separate transcription unit.
摘要
通过限制性内切酶图谱分析和酶活性测定，对一系列形成噬菌斑的metJB转导噬菌体的结构进行了研究。其中一种噬菌体λpmet100在EDTA存在下经热休克处理后失活，并从存活者中筛选出缺失突变体。利用其中两个突变体λpmet100 delta 1和λpmet100 delta 2，在大肠杆菌K-12的连锁图谱上顺时针移动时，证实了基因顺序为metJ metB。其他结果表明，metB基因可以独立于met调节子的任何其他组分进行表达，并且metJ基因也形成一个单独的转录单元。
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