Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3601-5. doi: 10.1073/pnas.82.11.3601.
The regulation of the expression of the Escherichia coli metF gene, which codes for 5,10-methylenetet-rahydrofolate reductase (EC 1.1.99.15), has been investigated by using a simplified DNA-directed in vitro system that measures the formation of the first dipeptide (fMet-Ser) of the gene product. The synthesis of fMet-Ser directed by a plasmid containing the metF gene is specifically inhibited by metJ protein (repressor protein). S-Adenosylmethionine enhances the inhibition by the metJ protein of metF gene expression. The inhibition by the metJ protein is at the level of transcription and the results suggest that S-adenosylmethionine is functioning as an allosteric effector.
已使用简化的 DNA 指导的体外系统研究了编码 5,10-亚甲基四氢叶酸还原酶（EC 1.1.99.15）的大肠杆菌 metF 基因表达的调节。该系统可测量基因产物的第一个二肽（甲硫氨酰-丝氨酸）的形成。含有 metF 基因的质粒指导的 fMet-Ser 合成被 metJ 蛋白（抑制蛋白）特异性抑制。S-腺苷甲硫氨酸增强了 metJ 蛋白对 metF 基因表达的抑制作用。该抑制作用发生在转录水平，结果表明 S-腺苷甲硫氨酸作为别构效应物发挥作用。
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