Loss of p53 Expression in Gastric Epithelial Cells of -Infected Jordanian Patients.

BACKGROUND

Around half of the global population is chronically infected with the stomach bacterium making it one of the most common chronic infections worldwide. induces the production of reactive oxygen species, DNA damage, and accelerates the degradation of the tumor suppressor protein p53, which may lead to cancer development. In this study, we investigated the relationship between infection and the expression of p53 in gastric mucosa in a group of patients from Jordan.

METHODS

In this retrospective case-control study, the epithelium of gastric glands in subjects chronically infected with was examined for the expression of p53. Paraffin-embedded gastric biopsy samples from the archives for 50 Jordanian patients diagnosed with chronic infection and 25 samples free of infection and any other gastric abnormalities were selected. Samples were analyzed for the presence of as well as p53 expression levels in the mucosa and submucosa by immunohistochemical analyses and Western blotting.

RESULTS

was detected in the gastric tissues of infected individuals ( = 50); whereas, no infection was detected in uninfected healthy individuals ( = 25) using immunohistochemistry. In contrast to the noninfected samples of gastric mucosa, no nuclear p53 expression was detected in the infected samples using immunohistochemistry. In addition, the levels of p53 in -positive samples detected by Western blotting were significantly lower than those in the negative individuals.

CONCLUSION

Our data reveal that p53 protein expression decreased in gastric mucosa of patients infected with . The loss of this tumor suppressor may play a role in the increased risk for tumor initiation associated with carriage.