年龄相关性黄斑变性和视网膜静脉阻塞中鸢尾素和炎症细胞因子的变化。
The Changes of Irisin and Inflammatory Cytokines in the Age-Related Macular Degeneration and Retinal Vein Occlusion.
机构信息
Department of Ophthalmology, Peking University People's Hospital; Eye Diseases and Optometry Institute; Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases; College of Optometry, Peking University Health Science Center, Beijing, China.
Department of Ophthalmology, Peking University International Hospital, Beijing, China.
出版信息
Front Endocrinol (Lausanne). 2022 Mar 17;13:861757. doi: 10.3389/fendo.2022.861757. eCollection 2022.
PURPOSE
Age-related macular degeneration (AMD) and retinal vein occlusion (RVO) are irreversible chorioretinal diseases, which might induce severe damage in visual function. The metabolic factor and inflammatory factors might play important roles in the pathogenesis of AMD and RVO. The levels of irisin and 14 cytokines were analyzed in aqueous humor of AMD and RVO eyes to evaluate the roles of irisin and inflammatory factors.
METHODS
We collected aqueous humor samples from patients with AMD ( = 27), RVO ( = 30), and cataract (as control, = 23) eyes. Samples were assayed using ELISA kit for irisin and a multiplex immunoassay kit for 14 cytokines. The macular thickness (MT) was measured with OCT in all included eyes.
RESULTS
MT in the RVO group is significantly higher than that in the AMD or control group. Irisin levels in the aqueous samples of AMD and RVO eyes were both significantly lower than that in the control. Furthermore, a positive correlation was found between irisin and MT in the RVO. Compared with the controls, AMD eyes had significantly higher levels of BDNF, VEGF-A, VEGF-R1, VEGF-R2, IL-10, TNF-α, VCAM-1, IP-10, and MCP-1. Similarly, RVO eyes had significantly higher levels of BDNF, VEGF-A, VEGF-R1, VEGF-R2, IL-6, IL-8, IL-10, TNF-α, ICAM-1, VCAM-1, IP-10, and MCP-1. However, there was no significant difference between the levels of PDGF-BB or TNF-β in these three groups. A negative correlation was found between VEGF-A and MT in AMD, as well as in control. Furthermore, a positive correlation was found between IL-6 and MT in the 80 included eyes, as well as in RVO. A positive correlation was found between ICAM-1 and MT in the 80 included eyes, as well as in RVO.
CONCLUSIONS
The metabolic factor, irisin levels in the aqueous humor are decreased in AMD and RVO eyes and show a positive correlation between irisin and MT in RVO eyes, prompting researchers to explore the relationship between irisin and macular edema. We also identified the higher expression of vascular growth factors (VEGF-A, VEGF-R1, and PDGF-BB), inflammatory cytokines (IL-6, IL-8, IL-10, and TNF-α), and chemokines (ICAM-1, VCAM-1, IP-10, and MCP-1) in AMD and RVO eyes.
目的
年龄相关性黄斑变性（AMD）和视网膜静脉阻塞（RVO）是不可逆转的脉络膜视网膜疾病，可能导致严重的视力损害。代谢因子和炎症因子可能在 AMD 和 RVO 的发病机制中发挥重要作用。分析 AMD 和 RVO 眼房水中鸢尾素和 14 种细胞因子的水平，以评估鸢尾素和炎症因子的作用。
方法
我们收集了 27 例 AMD 患者、30 例 RVO 患者和 23 例白内障（作为对照）患者的房水样本。使用 ELISA 试剂盒检测房水中鸢尾素的水平，使用多重免疫分析试剂盒检测 14 种细胞因子的水平。所有纳入的眼均采用 OCT 测量黄斑厚度（MT）。
结果
RVO 组的 MT 明显高于 AMD 组或对照组。AMD 和 RVO 眼房水中的鸢尾素水平均明显低于对照组。此外，RVO 中鸢尾素与 MT 呈正相关。与对照组相比，AMD 眼的 BDNF、VEGF-A、VEGF-R1、VEGF-R2、IL-10、TNF-α、VCAM-1、IP-10 和 MCP-1 水平明显升高。同样，RVO 眼的 BDNF、VEGF-A、VEGF-R1、VEGF-R2、IL-6、IL-8、IL-10、TNF-α、ICAM-1、VCAM-1、IP-10 和 MCP-1 水平明显升高。然而，这三组 PDGF-BB 或 TNF-β 的水平无明显差异。AMD 眼和对照组眼的 VEGF-A 与 MT 呈负相关。此外，80 只眼的 IL-6 与 MT 呈正相关，RVO 眼也呈正相关。80 只眼的 ICAM-1 与 MT 呈正相关，RVO 眼也呈正相关。
结论
代谢因子，房水中的鸢尾素水平在 AMD 和 RVO 眼中降低，并且在 RVO 眼中，鸢尾素与 MT 之间呈正相关，提示研究人员探索鸢尾素与黄斑水肿之间的关系。我们还发现 AMD 和 RVO 眼中血管生长因子（VEGF-A、VEGF-R1 和 PDGF-BB）、炎症细胞因子（IL-6、IL-8、IL-10 和 TNF-α）和趋化因子（ICAM-1、VCAM-1、IP-10 和 MCP-1）表达升高。
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