Institute of Chinese Sturgeon Disease, Yangtze University, Jingzhou, China.
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang, China.
Front Immunol. 2022 Mar 17;13:854689. doi: 10.3389/fimmu.2022.854689. eCollection 2022.
To further study the biological function of interferon-gamma (IFN-γ) in the Chinese sturgeon (), we conducted a transcriptome analysis of primary macrophages induced by IFN-γ using Illumina sequencing technology. We obtained 88,879 unigenes, with a total length of 93,919,393 bp, and an average length of 1,057bp. We identified 8,490 differentially expressed genes (DEGs) between the untreated and IFN-γ-treated macrophages, with 4,599 upregulated and 3,891 downregulated. Gene ontology (GO) analysis showed that 4,044 DEGs were enriched in the biological, cellular components, and molecular function categories. Kyoto Encyclopedia of Genes and Genomes (KEGG) identified 278 immunity-related pathways enriched for the DEGs. According to the GO enrichment results, eight key immunity-related genes were screened for verification using qPCR. Results indicate that IFN-γ can activate macrophage Interferon Regulatory Factors (IRFs) and type I interferon (IFN-I), activate RIG-I-like and Toll-like receptor-related pathways, and improve the antiviral ability of macrophages in Chinese sturgeon.
为了进一步研究干扰素-γ（IFN-γ）在中华鲟中的生物学功能，我们使用 Illumina 测序技术对 IFN-γ诱导的原代巨噬细胞进行了转录组分析。我们获得了 88879 个 unigenes，总长度为 93919393bp，平均长度为 1057bp。我们鉴定了未处理和 IFN-γ处理的巨噬细胞之间的 8490 个差异表达基因（DEGs），其中 4599 个上调，3891 个下调。基因本体论（GO）分析表明，4044 个 DEGs 富集在生物、细胞成分和分子功能类别中。京都基因与基因组百科全书（KEGG）鉴定了 278 个与免疫相关的途径，这些途径富集了 DEGs。根据 GO 富集结果，使用 qPCR 筛选了 8 个关键的免疫相关基因进行验证。结果表明，IFN-γ 可以激活巨噬细胞干扰素调节因子（IRFs）和 I 型干扰素（IFN-I），激活 RIG-I 样和 Toll 样受体相关途径，提高中华鲟巨噬细胞的抗病毒能力。
