Abdinghoff Jan, Servello Davide, Jacobs Tobias, Beckmann Anja, Tschernig Thomas
Institute of Anatomy and Cell Biology, Saarland University, Medical Campus, D-66424 Homburg/Saar, Germany.
Biomed Rep. 2022 May;16(5):42. doi: 10.3892/br.2022.1525. Epub 2022 Mar 22.
The TRPC6 channel is permeable to calcium ions as well as other ions and plays an important role in the physiology and pathophysiology of vessels. Findings from animal and cell culture experiments have shown its involvement in important vascular processes such as the Bayliss effect or endothelial-mediated vasodilatation. Furthermore, the relevance of TRPC6 channels in humans has become apparent based on diseases such as idiopathic pulmonary arterial hypertension, focal segmental glomerulosclerosis and atherosclerosis, amongst others. However, histological evidence that systematically detects TRPC6 channels in human vessels has not been provided to date. In this study, 40 vessel sections from nine body donors were obtained, processed and stained with a knockout-validated antibody against the TRPC6 protein using immunohistochemistry and western blotting. More than half of the samples yielded evidence of TRPC6 channel expression in the intima and adventitia. TRPC6 channels were detected in the tunica media in only one of 40 cases. TRPC6 detection in the human intima confirmed several demonstrated physiological aspects of the TRPC6 channels in the vasculature and may also be involved in associated human diseases. The near absence of TRPC6 channels in the tunica media was in contrast to a view that is primarily based on animal studies, from which its presence was assumed.
瞬时受体电位阳离子通道6（TRPC6）对钙离子以及其他离子具有通透性，在血管的生理和病理生理过程中发挥重要作用。动物实验和细胞培养实验结果表明，它参与了诸如贝利斯效应或内皮介导的血管舒张等重要的血管过程。此外，基于特发性肺动脉高压、局灶节段性肾小球硬化和动脉粥样硬化等疾病，TRPC6通道在人类中的相关性也已显现。然而，迄今为止尚未提供系统检测人类血管中TRPC6通道的组织学证据。在本研究中，从9名尸体供体获取了40个血管切片，进行处理，并使用免疫组织化学和蛋白质印迹法，用针对TRPC6蛋白的经过敲除验证的抗体进行染色。超过一半的样本在内膜和外膜中显示出TRPC6通道表达的证据。在40个病例中，只有1例在中膜检测到TRPC6通道。在人类内膜中检测到TRPC6证实了TRPC6通道在脉管系统中的几个已证实的生理方面，并且可能也与相关人类疾病有关。中膜中几乎不存在TRPC6通道，这与主要基于动物研究得出的认为其存在的观点形成了对比。
