Raming Cedric, Meier Carola, Tschernig Thomas
Institute of Anatomy and Cell Biology, Saarland University, Medical Campus, 66424 Homburg, Germany.
NeuroSci. 2025 May 19;6(2):44. doi: 10.3390/neurosci6020044.
Since its discovery, TRPC6 has been associated with a variety of physiological and pathophysiological processes in different tissues. It functions as a non-selective cation channel and belongs to the group of TRP channels. Its importance in the development of pain hypersensitivity is becoming increasingly apparent. This condition has already been associated with increased expression of TRPC6 in dorsal root ganglia. Apart from the fact that most of the evidence has been obtained from samples of animal origin, it remains unclear whether the channel is also expressed in peripheral nerves outside the dorsal root ganglia. The aim of this work was therefore to examine peripheral nerves from human samples for TRPC6. For this purpose, samples of both the sciatic and ulnar nerves were taken from a total of eight body donors and analyzed by immunohistochemistry. Both longitudinal and transverse sections were obtained from the samples and stained. In total, 43 of 48 histological sections showed a positive immunosignal. There were no major differences between the sciatic and ulnar nerves with regard to staining. There was a slight difference in the staining intensity of transverse and longitudinal sections. The longitudinal sections of both nerves were consistently colored slightly more intensely. However, the inter-individual differences between the donors were more pronounced. Interestingly, the samples of a donor who suffered from chronic pain syndrome during his lifetime were particularly strongly stained. This is consistent with the knowledge gained to date, largely from animal experiments, that the channel shows increased expression in pain conditions in dorsal root ganglia. In the future, TRPC6 could therefore be a target in pain therapy.
自发现以来，TRPC6已与不同组织中的多种生理和病理生理过程相关联。它作为一种非选择性阳离子通道发挥作用，属于瞬时受体电位（TRP）通道家族。其在痛觉过敏发展中的重要性日益明显。这种情况已与背根神经节中TRPC6表达增加相关。除了大多数证据来自动物源性样本这一事实外，尚不清楚该通道是否也在背根神经节以外的周围神经中表达。因此，这项工作的目的是检测人类样本中的周围神经是否存在TRPC6。为此，从总共八名尸体供体身上采集了坐骨神经和尺神经样本，并通过免疫组织化学进行分析。从样本中获取纵向和横向切片并进行染色。在总共48个组织学切片中，有43个显示出阳性免疫信号。坐骨神经和尺神经在染色方面没有重大差异。横向和纵向切片的染色强度存在轻微差异。两条神经的纵向切片染色始终略深。然而，供体之间的个体差异更为明显。有趣的是，一名生前患有慢性疼痛综合征的供体的样本染色特别强烈。这与迄今为止主要从动物实验中获得的知识一致，即该通道在背根神经节的疼痛状态下表达增加。因此，未来TRPC6可能成为疼痛治疗的一个靶点。
