Daum Felix, Flockerzi Fidelis, Bozzato Alessandro, Schick Bernhard, Tschernig Thomas
Institute of Anatomy and Cell Biology, Faculty of Medicine, Saarland University, D-66421 Homburg/Saar, Germany.
Institute of Pathology, Faculty of Medicine, Saarland University, D-66421 Homburg/Saar, Germany.
Med Int (Lond). 2024 Aug 2;4(6):62. doi: 10.3892/mi.2024.186. eCollection 2024 Nov-Dec.
Transient receptor potential canonical channel 6 (TRPC6) is a non-selective cation channel that is activated by diacylglycerol. It belongs to the TRP superfamily, is expressed in numerous tissues and has been shown to be associated with diseases, such as focal segmental glomerulosclerosis, idiopathic pulmonary arterial hypertension and cardiac hypertrophy. The investigation of the channel in human lymphoid tissues has thus far been limited to mRNA analysis or the western blotting of isolated lymphoid cell lines. The present study aimed to detect the channel in human lymphoid tissue using immunohistochemistry. For this purpose, lymphatic tissues were obtained from body donors. The lymphatic organs analyzed included the lymph nodes, spleen, palatine tonsil, gut-associated lymphoid tissues (ileum and vermiform appendix) and thymus. A total of 102 samples were obtained and processed for hematoxylin and eosin (H&E) staining. The H&E staining method was employed to identify five samples with good morphology. In total, three samples of the palatine tonsil of patients were included. Immunostaining was carried out using a knockout-validated anti-TRPC6 antibody. As shown by the results, using immunohistochemical staining, the presence of TRPC6 was confirmed in all the analyzed lymphatic tissue samples. Lymphocytes in lymph nodes, spleen, palatine tonsil, thymus, and gut-associated lymphatic tissues in ileum and vermiform appendix exhibited a positive staining signal. The follicle-associated epithelium of the palatine tonsil, ileum and appendix also demonstrated staining. Vessels of the lymphatic organs, particularly the trabecular arteries of the spleen, the submucosal vessels of the appendix and ileum, as well as the high endothelial venules in the palatine tonsils and lymphatic vessels of the lymph nodes expressed TRPC6 protein. TRPC6 in follicles may be involved in the immune response. TRPC6 in high endothelial venules suggests a role in leukocyte migration. The role of TRPC6 and other channels of the TRP family in lymphatic organs warrant further investigations to elucidate whether TRP channels are a pharmacological target.
瞬时受体电位香草酸亚型6（TRPC6）是一种非选择性阳离子通道，可被二酰基甘油激活。它属于瞬时受体电位（TRP）超家族，在多种组织中表达，并已显示与多种疾病相关，如局灶节段性肾小球硬化、特发性肺动脉高压和心肌肥大。迄今为止，对该通道在人类淋巴组织中的研究仅限于mRNA分析或对分离的淋巴细胞系进行蛋白质印迹分析。本研究旨在使用免疫组织化学方法检测人类淋巴组织中的该通道。为此，从遗体捐献者身上获取淋巴组织。分析的淋巴器官包括淋巴结、脾脏、腭扁桃体、肠道相关淋巴组织（回肠和阑尾）和胸腺。共获取102个样本并进行苏木精和伊红（H&E）染色处理。采用H&E染色方法鉴定出5个形态良好的样本。总共纳入了3例患者的腭扁桃体样本。使用经过敲除验证的抗TRPC6抗体进行免疫染色。结果显示，通过免疫组织化学染色，在所有分析的淋巴组织样本中均证实存在TRPC6。淋巴结、脾脏、腭扁桃体、胸腺以及回肠和阑尾中的肠道相关淋巴组织中的淋巴细胞呈现阳性染色信号。腭扁桃体、回肠和阑尾的滤泡相关上皮也显示出染色。淋巴器官的血管，特别是脾脏的小梁动脉、阑尾和回肠的黏膜下血管，以及腭扁桃体中的高内皮微静脉和淋巴结的淋巴管均表达TRPC6蛋白。滤泡中的TRPC6可能参与免疫反应。高内皮微静脉中的TRPC6表明其在白细胞迁移中发挥作用。TRPC6以及TRP家族的其他通道在淋巴器官中的作用值得进一步研究，以阐明TRP通道是否为药理学靶点。
