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RNA测序分析揭示了HVCN1质子通道在心脏pH稳态中的作用。

RNA-Seq Analyses Reveal Roles of the HVCN1 Proton Channel in Cardiac pH Homeostasis.

作者信息

Wu Xin, Li Yawei, Maienschein-Cline Mark, Feferman Leonid, Wu Longjun, Hong Liang

机构信息

Department of Medicine, University of Illinois at Chicago, Chicago, IL, United States.

Department of Preventive Medicine, Northwestern University, Chicago, IL, United States.

出版信息

Front Cell Dev Biol. 2022 Mar 16;10:860502. doi: 10.3389/fcell.2022.860502. eCollection 2022.

DOI:10.3389/fcell.2022.860502
PMID:35372367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8967321/
Abstract

The voltage-gated proton channel HVCN1 is a member of the voltage-gated ion channel family. HVCN1 channel controls acid extrusion and regulates pH homeostasis in various cell types. Recent evidence indicated that the HVCN1 channel was associated with cardiac function. To investigate the role of HVCN1 in cardiac myocytes, we performed an RNA sequencing analysis of murine hearts and showed that HVCN1 null hearts exhibited a differential transcriptome profile compared with wild-type hearts. The RNA-seq data indicating impaired pH homeostasis in HVCN1 null hearts were the downregulated NADPH oxidoreductases (NOXs) and decreased expression of Cl/HCO exchanger, indicating HVCN1 is a regulator of gene transcriptional networks controlling NOX signaling and CO homeostasis in the heart. Additionally, HVCN1 null hearts exhibited differential expression of cardiac ion channels, suggesting a potential role of HVCN1 in cardiac electrophysiological remodeling. The study highlights the importance of HVCN1 in cardiac function and may present a novel target associated with heart diseases.

摘要

电压门控质子通道HVCN1是电压门控离子通道家族的成员。HVCN1通道控制酸的排出并调节多种细胞类型中的pH稳态。最近的证据表明，HVCN1通道与心脏功能有关。为了研究HVCN1在心肌细胞中的作用，我们对小鼠心脏进行了RNA测序分析，结果表明，与野生型心脏相比，HVCN1基因敲除的心脏表现出不同的转录组谱。RNA测序数据表明，HVCN1基因敲除的心脏中pH稳态受损，这表现为NADPH氧化还原酶（NOXs）下调以及Cl/HCO交换体表达降低，这表明HVCN1是控制心脏中NOX信号传导和CO稳态的基因转录网络的调节因子。此外，HVCN1基因敲除的心脏表现出心脏离子通道的差异表达，这表明HVCN1在心脏电生理重塑中具有潜在作用。该研究突出了HVCN1在心脏功能中的重要性，并可能提出一个与心脏病相关的新靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/e6b2d78498b8/fcell-10-860502-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/6757cf26120c/fcell-10-860502-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/22b06d5a4672/fcell-10-860502-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/edea29da1652/fcell-10-860502-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/14c5e48310a2/fcell-10-860502-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/3b7ff91d008b/fcell-10-860502-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/c3bcc9733661/fcell-10-860502-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/e6b2d78498b8/fcell-10-860502-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/6757cf26120c/fcell-10-860502-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/22b06d5a4672/fcell-10-860502-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/edea29da1652/fcell-10-860502-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/14c5e48310a2/fcell-10-860502-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/3b7ff91d008b/fcell-10-860502-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/c3bcc9733661/fcell-10-860502-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbeb/8967321/e6b2d78498b8/fcell-10-860502-g007.jpg

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