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HVCN1 通道对于猪精子体外获能过程中维持精子活力有关。

HVCN1 Channels Are Relevant for the Maintenance of Sperm Motility During In Vitro Capacitation of Pig Spermatozoa.

机构信息

Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, E-17003 Girona, Spain.

Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, E-17003 Girona, Spain.

出版信息

Int J Mol Sci. 2020 May 4;21(9):3255. doi: 10.3390/ijms21093255.

DOI:10.3390/ijms21093255
PMID:32375375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7246839/
Abstract

The objective of the present study was to determine the physiological role of voltage-gated hydrogen channels 1 (HVCN1 channels) during in vitro capacitation of pig spermatozoa. Sperm samples from 20 boars were incubated in capacitating medium for 300 minutes (min) in the presence of 2-guanidino benzimidazole (2-GBI), a specific HVCN1-channel blocker, added either at 0 min or after 240 min of incubation. Control samples were incubated in capacitating medium without the inhibitor. In all samples, acrosomal exocytosis was triggered with progesterone after 240 min of incubation. Sperm viability, sperm motility and kinematics, acrosomal exocytosis, membrane lipid disorder, intracellular calcium levels and mitochondrial membrane potential were evaluated after 0, 60, 120, 180, 240, 250, 270 and 300 min of incubation. While HVCN1-blockage resulted in altered sperm viability, sperm motility and kinematics and reduced mitochondrial membrane potential as compared to control samples, at any blocker concentration and incubation time, it had a non-significant effect on intracellular Ca levels determined through Fluo3-staining. The effects on acrosomal exocytosis were only significant in blocked samples at 0 min, and were associated with increased membrane lipid disorder and Ca levels of the sperm head determined through Rhod5-staining. In conclusion, HVCN1 channels play a crucial role in the modulation of sperm motility and kinematics, and in Ca entrance to the sperm head.

摘要

本研究旨在确定电压门控氢通道 1（HVCN1 通道）在猪精子体外获能过程中的生理作用。将 20 头公猪的精子样本在获能培养基中孵育 300 分钟（min），在存在 2-胍基苯并咪唑（2-GBI）的情况下，2-GBI 是一种特异性的 HVCN1 通道阻断剂，要么在孵育 0 分钟时添加，要么在孵育 240 分钟后添加。对照样品在没有抑制剂的获能培养基中孵育。在所有样品中，在孵育 240 分钟后用孕酮触发顶体反应。在孵育 0、60、120、180、240、250、270 和 300 min 后，评估精子活力、精子运动和运动学、顶体反应、膜脂紊乱、细胞内钙水平和线粒体膜电位。与对照样品相比，HVCN1 阻断导致精子活力、精子运动和运动学改变和线粒体膜电位降低，但在任何阻断剂浓度和孵育时间下，对通过 Fluo3 染色确定的细胞内 Ca 水平均无显著影响。对顶体反应的影响仅在 0 分钟时的阻断样品中具有统计学意义，并且与通过 Rhod5 染色确定的精子头部增加的膜脂紊乱和 Ca 水平相关。总之，HVCN1 通道在调节精子运动和运动学以及 Ca 进入精子头部方面起着至关重要的作用。

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6
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