Vandenbol M, Durand P, Bolle P A, Dion C, Portetelle D, Hilger F
Unité de Microbiologie, Faculté des Sciences Agronomiques de Gembloux, Belgium.
Yeast. 1994 Dec;10(12):1657-62. doi: 10.1002/yea.320101216.
We have sequenced on both strands a 40,257 bp fragment located near the left telomere of chromosome X of Saccharomyces cerevisiae. The sequenced segment contains 21 open reading frames (ORFs) at least 100 amino acids long. Five of the ORFs correspond to known amino acid sequences: two hypothetical proteins in the subtelomeric Y' repeat region of 65.4 and 12.8 KDa, the cytochrome B pre-mRNA processing CBP1 protein, the mitochondrial nuclease NUC1 and the CRT1 protein. Of the 16 remaining ORFs, eight show highest homologies with the S. cerevisiae hexose transporters family (two ORFs), the yeast alpha-glucosidase (two ORFs), the yeast PEP1 precursor, the Escherichia coli galactoside O-acetyltransferase, the S. cerevisiae 137.7 KDa protein located in the Y' region and a protein of unknown function of Schizosaccharomyces pombe. Finally, eight of the ORFs exhibit no significant similarity with any amino acid sequences described in data banks. DNA sequence comparison has revealed the presence of different repeated elements characteristic of yeast chromosome ends. Disruption studies have been performed on two ORFs encoding putative proteins of unknown function.
我们对位于酿酒酵母X染色体左端粒附近的一段40257 bp的片段进行了双链测序。测序片段包含21个开放阅读框（ORF），长度至少为100个氨基酸。其中5个ORF与已知氨基酸序列相对应：65.4 kDa和12.8 kDa的亚端粒Y'重复区域中的两种假设蛋白、细胞色素B前体mRNA加工CBP1蛋白、线粒体核酸酶NUC1和CRT1蛋白。在其余16个ORF中，8个与酿酒酵母己糖转运蛋白家族（两个ORF）、酵母α-葡萄糖苷酶（两个ORF）、酵母PEP1前体、大肠杆菌半乳糖苷O-乙酰基转移酶、位于Y'区域的酿酒酵母137.7 kDa蛋白以及粟酒裂殖酵母的一种功能未知的蛋白具有最高同源性。最后，8个ORF与数据库中描述的任何氨基酸序列均无显著相似性。DNA序列比较揭示了酵母染色体末端特有的不同重复元件的存在。已对两个编码功能未知的假定蛋白的ORF进行了中断研究。
