Department of Biotechnology, The Catholic University of Korea, Bucheon-si, Gyeonggi-do 14662, Republic of Korea.
Department of Chemical Engineering and Materials Science, Graduate Program in System Health Science & Engineering, Ewha Womans University, Seoul 03760, Republic of Korea.
Bioresour Technol. 2022 May;352:127106. doi: 10.1016/j.biortech.2022.127106. Epub 2022 Apr 1.
Lignin valorization depends on microbial upcycling of various aromatic compounds in the form of a complex mixture, including p-coumaric acid and ferulic acid. In this study, an engineered Pseudomonas putida strain utilizing lignin-derived monomeric compounds via biological funneling was developed to produce 2-pyrone-4,6-dicarboxylic acid (PDC), which has been considered a promising building block for bioplastics. The biosynthetic pathway for PDC production was established by introducing the heterologous ligABC genes under the promoter P in a strain lacking pcaGH genes to accumulate a precursor of PDC, i.e., protocatechuic acid. Based on the culture optimization, fed-batch fermentation of the final strain resulted in 22.7 g/L PDC with a molar yield of 1.0 mol/mol and productivity of 0.21 g/L/h. Subsequent purification of PDC at high purity was successfully implemented, which was consequently applied for the novel polyester.
木质素的增值取决于微生物对各种芳香族化合物的循环利用，这些化合物以复杂混合物的形式存在，包括对香豆酸和阿魏酸。在本研究中，开发了一种利用木质素衍生的单体化合物通过生物流实现的工程化假单胞菌菌株，以生产 2-吡喃酮-4,6-二羧酸（PDC），PDC 已被认为是一种有前途的生物塑料构建块。通过在缺乏 pcaGH 基因的菌株中引入异源 ligABC 基因，并在 P 启动子下表达，建立了 PDC 的生物合成途径，以积累 PDC 的前体，即原儿茶酸。基于培养优化，最终菌株的分批补料发酵得到了 22.7 g/L 的 PDC，摩尔产率为 1.0 mol/mol，生产强度为 0.21 g/L/h。成功实现了 PDC 的高纯度纯化，随后将其用于新型聚酯的生产。
