Otsuka Yuichiro, Araki Takuma, Suzuki Yuzo, Nakamura Masaya, Kamimura Naofumi, Masai Eiji
Department of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
Department of Forest Resource Chemistry, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
Bioresour Technol. 2023 Jun;377:128956. doi: 10.1016/j.biortech.2023.128956. Epub 2023 Mar 23.
2-Pyrone-4,6-dicarboxylic acid (PDC) is a valuable building block molecule produced from lignin-derived aromatic compounds by biological funneling. This study aimed to design a fermentation process for producing PDC from vanillic acid, which could be applied at an industrial production. Metabolomic analysis revealed that a high primary metabolic activity within cells was required to improve the production efficiency. Moreover, a medium with ammonium salts and no alkali metals was advantageous because it suppressed the formation of PDC-metal complexes. Resulting optimized process yielded the highest PDC titer and productivity ever reported: 99.9 g/L and 1.69 g/L/h, respectively. Per batch, 190 g of PDC was produced per liter of initial culture media, and the final liquid volume was 1.9 L. This study demonstrates the design of fermentation processes for the advanced industrial utilization of lignin by biological funneling.
2-吡喃酮-4,6-二羧酸（PDC）是一种通过生物转化从木质素衍生的芳香族化合物中产生的重要基础分子。本研究旨在设计一种从香草酸生产PDC的发酵工艺，该工艺可应用于工业生产。代谢组学分析表明，提高细胞内的初级代谢活性对于提高生产效率是必要的。此外，含有铵盐且不含碱金属的培养基是有利的，因为它抑制了PDC-金属络合物的形成。优化后的工艺产生了有史以来报道的最高PDC滴度和生产率：分别为99.9 g/L和1.69 g/L/h。每批每升初始培养基可生产190 g PDC，最终液体体积为1.9 L。本研究展示了通过生物转化对木质素进行先进工业利用的发酵工艺设计。
