Wang Shaodan, Xie Shaolin, Zhang Chaonan, Pan Zhengkun, Sun Di, Zhou Aiguo, Xu Guohuan, Zou Jixing
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Fish Shellfish Immunol. 2022 May;124:74-81. doi: 10.1016/j.fsi.2022.03.045. Epub 2022 Apr 2.
The interaction between microplastics and contaminants has potentially generated new undefined risks on animals and ecosystems, and nano-microplastics are considered to be more harmful than microplastics. This experiment investigated the interactions and effects of nano-microplastics with heavy metals cadmium in hybrid snakehead. Different concentrations of nano-microplastics 80 nm (50 μg/L and 500 μg/L) and Cd (50 μg/L) were used for exposure, and four sampling points were set for 24 h, 48 h, 96 h and clear-48 h. Results indicated that the morphology of gill was altered under the influence of nano-microplastics and cadmium, and the damage was aggravated with time. Nano-microplastics and Cd can cause oxidative damage to fish liver partly by effect the activities of antioxidant enzyme, and significantly suppressed the expressions of genes related to the inflammation (IL-1β and TNF-α) and as well as significantly up-regulated the expression of genes HSP70 and SOD. Additionally, the mRNA levels of MT gene can be speculated that the heavy metal cadmium may accumulated in the body over time. And the concentration of heavy metals will also affect their accumulation in the body. Our study elucidated the nano-microplastics and Cd will increase the impact on environmental and organisms that the nano-microplastics contribute to the bioaccumulation of metals, which served as a new support for study the interaction between environmental contaminants.
微塑料与污染物之间的相互作用可能对动物和生态系统产生新的未知风险，并且纳米微塑料被认为比微塑料更具危害性。本实验研究了纳米微塑料与重金属镉在杂交鳢中的相互作用及影响。使用不同浓度的80纳米纳米微塑料（50微克/升和500微克/升）和镉（50微克/升）进行暴露实验，并设置了24小时、48小时、96小时和清除48小时四个采样点。结果表明，在纳米微塑料和镉的影响下，鳃的形态发生了改变，且损伤随时间加剧。纳米微塑料和镉可部分通过影响抗氧化酶活性对鱼肝造成氧化损伤，并显著抑制与炎症相关基因（IL-1β和TNF-α）的表达，同时显著上调HSP70和SOD基因的表达。此外，MT基因的mRNA水平可以推测重金属镉可能会随时间在体内蓄积。并且重金属的浓度也会影响它们在体内的蓄积。我们的研究阐明了纳米微塑料和镉会增加对环境和生物的影响，纳米微塑料有助于金属的生物累积，这为研究环境污染物之间的相互作用提供了新的支持。
