粗提物和馏分的抗炎和抗癌特性：基于生物测定的方法。

Antiinflammatory and Anticancer Properties of Crude Extracts and Fractions: A Bioassay-Guided Approach.

机构信息

Institute of Food Science and Nutrition, Bahauddin Zakariya University, Multan 60800, Pakistan.

School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia.

出版信息

Biomed Res Int. 2022 Mar 28;2022:2277417. doi: 10.1155/2022/2277417. eCollection 2022.

DOI:10.1155/2022/2277417

PMID:35386306

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8979695/

Abstract

The study was an extension of our earlier work on antiinflammatory and anticancer properties of fruit. We aimed to develop a bioassay guided multistep purification technique for producing bioactive fractions of crude extracts. Dried fruit powder was sequentially fractionated with 100% dichloromethane, 100% methanol (MeOH), and 50% MeOH. Active extracts were subjected to liquid-liquid partitioning followed by subfractionation using RP-HPLC. Antioxidant, antiinflammatory, and anticancer activities of the fruit extracts, and their potent fractions were evaluated , while identification of compounds from the bioactive fractions was performed by ESI-MS/MS analysis. The amount of the identified compounds present was confirmed using external standards adopting a simple, accurate, and rapid analytical HPLC method. The results showed that 100% and 50% MeOH extracts possessed bioactivity; one of which (the 50% MeOH extract) displayed potent activity in all bioassays. MeOH extract (50%) derived fraction C and hydroalcoholic fraction 5 (GAHAF5) were observed to possess higher antioxidant, antiinflammatory, and anticancer activity. IC of GAHAF5 against MCF-7, HEp-2, and NCI-H522 cancer cells was recorded as 26.2, 51.4, and 63 g/mL, respectively. ESI-MS/MS and HPLC analysis identified catechin, chlorogenic acid, caffeic acid, and morin as potential bioactive compounds in the GAHAF5 fraction with concentrations of 1230, 491, 957, and 130 g/g, respectively. The findings indicated that bioactive fractions possessed antiinflammatory activity and were cytotoxic against breast cancer, lung cancer, and laryngeal cancer cell lines.

摘要

本研究是我们早期关于水果抗炎和抗癌特性研究的延伸。我们旨在开发一种基于生物活性测定的多步纯化技术，以生产粗提物的生物活性级分。干燥的水果粉末依次用 100%二氯甲烷、100%甲醇（MeOH）和 50% MeOH 进行分步提取。活性提取物进行液液萃取，然后用反相高效液相色谱法（RP-HPLC）进行亚分级。评估了水果提取物及其有效级分的抗氧化、抗炎和抗癌活性，并通过 ESI-MS/MS 分析鉴定了生物活性级分中的化合物。采用简单、准确、快速的 HPLC 分析方法，通过外标法对存在的化合物的量进行了确认。结果表明，100%和 50% MeOH 提取物具有生物活性；其中一种（50% MeOH 提取物）在所有生物测定中均表现出较强的活性。MeOH 提取物（50%）衍生的 C 级分和水醇提取物 5（GAHAF5）被观察到具有较高的抗氧化、抗炎和抗癌活性。GAHAF5 对 MCF-7、HEp-2 和 NCI-H522 癌细胞的 IC 分别为 26.2、51.4 和 63μg/mL。ESI-MS/MS 和 HPLC 分析鉴定出 GAHAF5 级分中的儿茶素、绿原酸、咖啡酸和桑色素为潜在的生物活性化合物，浓度分别为 1230、491、957 和 130μg/g。研究结果表明，生物活性级分具有抗炎活性，对乳腺癌、肺癌和喉癌细胞系具有细胞毒性。