Li Jiangfeng, Wang Chunyi, Gao Guoxing, Yin Xing, Pu Ximing, Shi Bing, Liu Yang, Huang Zhongbing, Wang Juan, Li Jingtao, Yin Guangfu
College of Biomedical Engineering, Sichuan University, Chengdu, 610065, PR China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, PR China.
Bioact Mater. 2021 Dec 21;15:53-67. doi: 10.1016/j.bioactmat.2021.11.034. eCollection 2022 Sep.
It remains a challenge to achieve satisfactory balance between biodegradability and osteogenic capacity in biosynthetic bone grafts. In this study, we aimed to address this challenge by incorporating mesoporous bioactive glass (MBG) into poly(caprolactone-co-glycolide) (PGA-PCL) at gradient ratios. MBG/PGA-PCL (PGC/M) scaffolds with MBG incorporation ratio at 0, 10%, 25% and 40% (PGC/M0-40) were synthesized using a modified solvent casting-particulate leaching method, and their physiochemical and biological properties were comprehensively evaluated. PGC/M scaffolds exhibited highly perforated porous structure with a large-pore size of 300-450 μm, with ordered MBGs of around 6.0 nm mesopores size uniformly dispersed. The increase in MBG incorporation ratio significantly improved the scaffold surface hydrophilicity, apatite-formation ability and pH stability, increased the weight loss rate while insignificantly influenced the molecular chains degradation of PGA-PCL component, and facilitated the attachment, spreading, viability and proliferation of rat bone marrow stromal cells (rBMSCs) on scaffolds. Moreover, rBMSCs cultured on PGC/M10-40 scaffolds demonstrated enhanced ALP activity and osteogenesis-related gene expression in a MBG dose-dependent manner as compared with those cultured on PGC/M0 scaffolds. When implanted to the rat cranial bone defect, PGC/M25 and PGC/M40 scaffolds induced significantly better bone repair as compared to PGC/M0 and PGC/M10 scaffolds. Besides, the biodegradability of PGC/M scaffolds correlated with the MBG incorporation ratio. These data suggested this novel PGC/M scaffolds as promising bone repair biomaterial with highly tunable hydrophilicity, bioactivity, cytocompatibility, osteogenic activity as well as biodegradability.
在生物合成骨移植材料中，要在生物可降解性和成骨能力之间实现令人满意的平衡仍然是一项挑战。在本研究中，我们旨在通过将介孔生物活性玻璃（MBG）以梯度比例掺入聚（己内酯-共-乙交酯）（PGA-PCL）中来应对这一挑战。使用改良的溶剂浇铸-颗粒沥滤法合成了MBG掺入率为0%、10%、25%和40%（PGC/M0-40）的MBG/PGA-PCL（PGC/M）支架，并对其物理化学和生物学特性进行了全面评估。PGC/M支架呈现出高度多孔的结构，大孔尺寸为300-450μm，有序的MBG介孔尺寸约为6.0nm且均匀分散。MBG掺入率的增加显著提高了支架表面亲水性、磷灰石形成能力和pH稳定性，增加了失重率，同时对PGA-PCL组分的分子链降解影响不显著，并促进了大鼠骨髓基质细胞（rBMSCs）在支架上的附着、铺展、活力和增殖。此外，与在PGC/M0支架上培养的细胞相比，在PGC/M10-40支架上培养的rBMSCs以MBG剂量依赖的方式表现出增强的碱性磷酸酶（ALP）活性和成骨相关基因表达。当植入大鼠颅骨缺损处时，与PGC/M0和PGC/M10支架相比，PGC/M25和PGC/M40支架诱导的骨修复效果明显更好。此外，PGC/M支架的生物可降解性与MBG掺入率相关。这些数据表明，这种新型的PGC/M支架是一种有前景的骨修复生物材料，具有高度可调的亲水性、生物活性、细胞相容性、成骨活性以及生物可降解性。
