Sánchez-Romo Daniel, Hernández-Vásquez César I, Pereyra-Alférez Benito, García-García Jorge H
Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av Universidad S/N, 66455, San Nicolás de los Garza, Nuevo León, Mexico.
Noncoding RNA Res. 2022 Mar 18;7(2):89-97. doi: 10.1016/j.ncrna.2022.03.002. eCollection 2022 Jun.
Plant-derived miRNAs can be found in the human body after dietary intake, and they can affect post-transcriptional gene regulation in human. It is important to identify targets to determine the possible effects in human genes by using computational approach. In this study, 787 possible mRNAs human targets were predicted by 84 miRNAs of wheat. A total of 14 miRNAs were identified with individual binding to 33 mRNAs associated with schizophrenia, epilepsy, neurodevelopmental disorders, and various cancers, located in the 3'UTR of the mRNA. A functional enrichment was carried out, where the results showed associations to pathways such as dopaminergic synapse (hsa04728), and signaling pathways, significantly associated with the target genes. The prediction of target mRNAs in humans by wheat miRNAs, offer candidates that could facilitate the search and verification, which could be of relevance for future projects and therefor contribute in the therapeutic treatment of various human diseases.
植物来源的微小RNA在饮食摄入后可在人体中被发现，并且它们能够影响人类的转录后基因调控。通过计算方法识别靶点对于确定其对人类基因可能产生的影响很重要。在本研究中，从小麦的84个微小RNA预测出787个可能的人类mRNA靶点。总共鉴定出14个微小RNA，它们分别与位于mRNA 3'UTR区域的33个与精神分裂症、癫痫、神经发育障碍及各种癌症相关的mRNA结合。进行了功能富集分析，结果显示与多巴胺能突触（hsa04728）等通路以及与靶基因显著相关的信号通路存在关联。小麦微小RNA对人类靶mRNA的预测提供了有助于搜索和验证的候选物，这可能与未来的项目相关，从而有助于各种人类疾病的治疗。
