Generation of a CRF-Cre transgenic rat and the role of central amygdala CRF cells in nociception and anxiety-like behavior.

Corticotropin-releasing factor type-1 (CRF) receptors are critical to stress responses because they allow neurons to respond to CRF released in response to stress. Our understanding of the role of CRF-expressing neurons in CRF-mediated behaviors has been largely limited to mouse experiments due to the lack of genetic tools available to selectively visualize and manipulate CRF cells in rats. Here, we describe the generation and validation of a transgenic CRF-Cre-Tomato rat. We report that and mRNA expression are highly colocalized in both the central amygdala (CeA), composed of mostly GABAergic neurons, and in the basolateral amygdala (BLA), composed of mostly glutamatergic neurons. In the CeA, membrane properties, inhibitory synaptic transmission, and responses to CRF bath application in Tomato neurons are similar to those previously reported in GFP cells in CRFR1-GFP mice. We show that stimulatory DREADD receptors can be targeted to CeA CRF cells via virally delivered Cre-dependent transgenes, that transfected Cre/Tomato cells are activated by clozapine-n-oxide in vitro and in vivo, and that activation of these cells in vivo increases anxiety-like and nocifensive behaviors. Outside the amygdala, we show that Cre-Tomato is expressed in several brain areas across the brain, and that the expression pattern of Cre-Tomato cells is similar to the known expression pattern of CRF cells. Given the accuracy of expression in the CRF-Cre rat, modern genetic techniques used to investigate the anatomy, physiology, and behavioral function of CRF neurons can now be performed in assays that require the use of rats as the model organism.