Pratt J J
Ann Clin Biochem. 1986 May;23 ( Pt 3):251-76. doi: 10.1177/000456328602300305.
Using progesterone, testosterone, androstenedione, 11-oxoprogesterone and 11 beta-hydroxyprogesterone as models, a new form of isotope dilution assay has been developed. A known mass of deuterium-labelled steroid is added to the serum sample. High-performance liquid chromatography is used to separate endogenous steroid from its deuterium-labelled form. After separation, the two forms of the analyte are quantitated using conventional methods: radioimmunoassay, enzyme-linked immunoassay and, where the concentrations are high enough, ultraviolet light absorption. The ratio of the amounts of the two forms of the analyte is used to calculate the amount of unlabelled material in the original sample. The assay principle is quite general. A variety of high resolution methods are available to separate isotopic analogues of the same compound. A number of detection methods can be used to quantitate the separated isotopic forms. Extension of this principle to other fields of interest in bio-medicine is discussed briefly.
以孕酮、睾酮、雄烯二酮、11-氧代孕酮和11β-羟基孕酮为模型，开发了一种新形式的同位素稀释测定法。将已知质量的氘标记类固醇添加到血清样本中。使用高效液相色谱法将内源性类固醇与其氘标记形式分离。分离后，使用常规方法对两种形式的分析物进行定量：放射免疫测定法、酶联免疫测定法，以及在浓度足够高时的紫外光吸收法。两种形式的分析物的量的比值用于计算原始样品中未标记物质的量。该测定原理非常通用。有多种高分辨率方法可用于分离同一化合物的同位素类似物。可以使用多种检测方法对分离出的同位素形式进行定量。简要讨论了该原理在生物医学其他感兴趣领域的扩展。
