Divisão de Medicina Veterinária, Guarda Nacional Republicana (GNR), Rua Presidente Arriaga, 9, 1200-771, Lisbon, Portugal.
MED - Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investigação e Formação Avançada, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554, Évora, Portugal.
Sci Rep. 2022 Apr 7;12(1):5887. doi: 10.1038/s41598-022-09934-y.
Osteoarthritis (OA) is a disease with a high negative impact on patient's quality of life and a high financial burden. It is a source of chronic pain and affects all mammals, including humans and dogs. As the dog is a common model for translation research of human OA, and exploring spontaneous dog OA can improve the health and well-being of both humans and dogs. To describe the effect of the intra-articular administration of stanozolol in a naturally occurring canine OA model, forty canine (N = 40) hip joints were randomly assigned to receive stanozolol or saline (control). On treatment day and at 8, 15, 30, 90, and 180 days post-treatment, several evaluations were conducted: weight distribution, joint range of motion, thigh girth, digital thermography, and radiographic signs. Also, synovial fluid C-reactive protein and interleukin-1 levels were evaluated. Results from four Clinical Metrology Instruments was also gathered. Results were compared with Repeated Measures ANOVA, with a Huynh-Feldt correction, paired-samples t-test, or Wilcoxon signed-rank test, with p < 0.05. OA was graded as mild (90%), moderate (5%), and severe (5%), including both sexes. They had a mean age of 6.5 ± 2.4 years and a bodyweight of 26.7 ± 5.2 kg. No differences were found between groups at treatment day in all considered evaluations. Weight distribution showed significant improvements with stanozolol from 15 days (p < 0.05) up to 180 days (p < 0.01). Lower values during thermographic evaluation in both views taken and improved joint extension at 90 (p = 0.02) and 180 days (p < 0.01) were observed. Pain and function scores improved up to 180 days. In the control group, radiographic signs progressed, in contrast with stanozolol. The use of stanozolol was safe and produced significant improvements in weight-bearing, pain score, and clinical evaluations in a naturally occurring canine OA model.
骨关节炎（OA）是一种对患者生活质量有负面影响且经济负担高的疾病。它是慢性疼痛的来源，影响所有哺乳动物，包括人类和狗。由于狗是人类 OA 翻译研究的常见模型，探索自发性狗 OA 可以改善人类和狗的健康和福祉。为了描述关节内给予司坦唑醇对自然发生的犬 OA 模型的影响，将 40 个犬（N = 40）髋关节随机分配接受司坦唑醇或生理盐水（对照）。在治疗日和治疗后 8、15、30、90 和 180 天，进行了多项评估：体重分布、关节活动范围、大腿周长、数字热成像和放射照相迹象。还评估了滑液 C 反应蛋白和白细胞介素 1 水平。还收集了四个临床计量仪器的结果。结果通过重复测量方差分析、Huynh-Feldt 校正、配对样本 t 检验或 Wilcoxon 符号秩检验进行比较，p < 0.05。OA 分为轻度（90%）、中度（5%）和重度（5%），包括两性。它们的平均年龄为 6.5 ± 2.4 岁，体重为 26.7 ± 5.2 公斤。在所有考虑的评估中，治疗日时两组之间没有差异。体重分布显示司坦唑醇从 15 天（p < 0.05）到 180 天（p < 0.01）显著改善。在两个视图中都观察到热成像评估中的较低值，并且在 90 天（p = 0.02）和 180 天（p < 0.01）时关节伸展得到改善。疼痛和功能评分在 180 天内得到改善。在对照组中，与司坦唑醇相反，放射照相迹象进展。在自然发生的犬 OA 模型中，司坦唑醇的使用是安全的，并在负重、疼痛评分和临床评估方面产生了显著改善。
