Divisão de Medicina Veterinária, Guarda Nacional Republicana, Lisbon, Portugal.
Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investigação e Formação Avançada, Universidade de Évora, Évora, Portugal.
Am J Sports Med. 2021 Mar;49(3):599-608. doi: 10.1177/0363546520981558. Epub 2021 Jan 11.
Osteoarthritis (OA) represents a significant burden to societies, as it affects quality of life and performance and implies a large cost in terms of health care. Autologous platelets are a regenerative treatment modality for OA that are thought to be a potential stimulation of the natural healing cascade.
To describe the effect of the platelet concentrate V-PET in the management of OA in a naturally occurring canine model, using several outcome assessment modalities.
Controlled laboratory study.
A total of 40 joints of active working police dogs with hip OA were randomly assigned to a control group (CG) and a platelet concentrate group (PCG; treatment) and evaluated. At treatment day (T0) and 8, 15, 30, 90, and 180 days after treatment, weight distribution, joint range of motion at flexion and extension, thigh girth, digital thermography, radiographic signs, 4 clinical metrology instruments, and synovial fluid interleukin 1 and C-reactive protein levels were recorded. Results were compared using repeated-measures analysis of variance with a Huynh-Feldt correction, paired-samples test, or Wilcoxon signed rank test, with < .05.
Dogs were 6.5 ± 2.4 years old (mean ± SD) and had a body weight of 26.7 ± 5.2 kg. At T0, 32 (80%) joints were graded as having mild OA, 6 (15%) as moderate, and 2 (5%) as severe. No differences were found between groups at T0. Between the PCG and CG, the symmetry index showed significant improvements in the PCG from 8 days ( = .01) to 180 days ( = .01). Joint flexion also improved in the PCG up to 90 days ( < .05) and extension improved up to 180 days ( < .01). Several clinical metrology instrument scores also improved up to 90 to 180 days after treatment. In the CG, radiographic signs progressed, while the PCG showed some improved signs. In both groups, increasing body weight and age corresponded with worse clinical and laboratory findings.
A single injection of platelet concentrate had a positive effect, lasting up to 6 months, on several clinical, imaging, and laboratory signs in a naturally occurring canine OA model.
We characterized the effects of this platelet concentrate in dogs, considered the gold standard of the study of OA, with a group of working animals with similar high demands as athletes.
骨关节炎（OA）对社会造成了重大负担，因为它影响生活质量和表现，并在医疗保健方面带来了巨大的成本。自体血小板是一种治疗 OA 的再生疗法，被认为是对自然愈合级联的潜在刺激。
使用多种结果评估方法，描述血小板浓缩物 V-PET 在自然发生的犬 OA 模型中的管理效果。
对照实验室研究。
共有 40 个患有髋关节 OA 的现役警犬关节被随机分配到对照组（CG）和血小板浓缩物组（PCG；治疗）并进行评估。在治疗日（T0）以及治疗后 8、15、30、90 和 180 天，记录体重分布、屈伸关节活动度、大腿围度、数字热成像、放射影像学征象、4 种临床计量学仪器以及滑液白细胞介素 1 和 C 反应蛋白水平。使用重复测量方差分析与 Huynh-Feldt 校正、配对样本 t 检验或 Wilcoxon 符号秩检验进行比较， <.05。
狗的年龄为 6.5 ± 2.4 岁（均值 ± 标准差），体重为 26.7 ± 5.2 kg。在 T0，32 个（80%）关节被评为轻度 OA，6 个（15%）为中度，2 个（5%）为重度。T0 时两组间无差异。在 PCG 中，对称性指数从 8 天（ =.01）到 180 天（ =.01）有显著改善。PCG 的关节屈曲度也在 90 天内得到改善（ <.05），伸展度在 180 天内得到改善（ <.01）。多项临床计量学仪器评分在治疗后 90 至 180 天也有所改善。在 CG 中，放射影像学征象进展，而 PCG 则显示出一些改善迹象。在两组中，体重增加和年龄与临床和实验室发现的恶化相关。
在自然发生的犬 OA 模型中，单次注射血小板浓缩物对多项临床、影像学和实验室指标有积极影响，持续时间长达 6 个月。
我们描述了这种血小板浓缩物在狗中的作用，狗被认为是 OA 研究的金标准，并且是一组具有与运动员相似高要求的工作犬。
