Alves J C, Santos A, Jorge P, Lavrador C, Carreira L Miguel
Divisão de Medicina Veterinária, Guarda Nacional Republicana (GNR), Rua Presidente Arriaga, 9, 1200-771, Lisbon, Portugal.
MED - Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investigação e Formação Avançada, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554, Évora, Portugal.
BMC Musculoskelet Disord. 2020 Feb 27;21(1):127. doi: 10.1186/s12891-020-3140-9.
Osteoarthritis (OA) represents a significant burden to societies, as it affects quality of life, performance and poses a large healthcare cost. We aimed to describe the use of a single intra-articular (IA) injection of an autologous platelet therapy in the management of osteoarthritis (OA) in a naturally occurring canine model.
Fifteen police working dogs with bilateral hip OA were treated with 3 ml of platelet concentrate per hip joint, produced with the V-PET kit. Response to treatment was measured by the Canine Brief Pain Inventory (CBPI, divided in pain interference score - PIS, and Pain Severity Score - PSS), Liverpool Osteoarthritis in Dogs (LOAD), Canine Orthopedic Index (COI, divided in four dimensions: function, gait, stiffness and quality of life - QOL) and the Hudson Visual Analogue Scale (HVAS). Seven different time points were considered: T0 (before treatment), T1 (after 15 days), T2, T3, T4, T5 and T6 (after 1, 2, 3, 4 and 5 months respectively). Results from each evaluation moment were compared with T0 with a Paired Samples T-Test, and a p < 0.05 was set.
Significant differences were observed at T1 (p < 0.01 for HVAS, PSS, COI, Gait and QOL; p = 0.01 for PIS; p = 0.02 for Function; and p < 0.05 for Stiffness), T2 (p < 0.01 for PSS, PIS and Gait; p = 0.01 for COI; p = 0.02 for HVAS, Function and QOL; and p = 0.04 for Stiffness), T3 (p < 0.01 for HVAS, PSS, PIS, Function and Gait; p = 0.01 for COI; and p = 0.02 for QOL), T4 (p < 0.01 for PSS; p = 0.03 for PIS and Gait), T5 (p < 0.01 for COI, Function and Gait; p = 0.03 for PSS, PIS and Stiffness), T6 (p < 0.01 for PSS, Function and Gait; p = 0.04 for PIS; p < 0.05 for COI) and T7 (p < 0.01 for PSS, Function and Gait; p = 0.01 for COI; and p < 0.05 for PIS).
Autologous platelet therapy was used without apparent harm in the subjects. A single administration produced significant improvements, which lasted several months, and therefore warrants further study.
骨关节炎（OA）给社会带来了沉重负担，因为它会影响生活质量、身体机能，并产生高昂的医疗费用。我们旨在描述在自然发生的犬类模型中，单次关节内（IA）注射自体血小板疗法在骨关节炎（OA）治疗中的应用。
15只患有双侧髋关节OA的警犬，每只髋关节注射3毫升用V-PET试剂盒制备的血小板浓缩液。通过犬类简短疼痛量表（CBPI，分为疼痛干扰评分 - PIS和疼痛严重程度评分 - PSS）、犬类利物浦骨关节炎量表（LOAD）、犬类骨科指数（COI，分为四个维度：功能、步态、僵硬程度和生活质量 - QOL）以及哈德逊视觉模拟量表（HVAS）来衡量治疗反应。考虑了七个不同的时间点：T0（治疗前）、T1（15天后）、T2、T3、T4、T5和T6（分别在1、2、3、4和5个月后）。每个评估时刻的结果与T0进行配对样本t检验，设定p < 0.05。
在T1（HVAS、PSS、COI、步态和QOL的p < 0.01；PIS的p = 0.01；功能的p = 0.02；僵硬程度的p < 0.05）、T2（PSS、PIS和步态的p < 0.01；COI的p = 0.01；HVAS、功能和QOL的p = 0.02；僵硬程度的p = 0.04）、T3（HVAS、PSS、PIS、功能和步态的p < 0.01；COI的p = 0.01；QOL的p = 0.02）、T4（PSS的p < 0.01；PIS和步态的p = 0.03）、T5（COI、功能和步态的p < 0.01；PSS、PIS和僵硬程度的p = 0.03）、T6（PSS、功能和步态的p < 0.01；PIS的p = 0.04；COI的p < 0.05）和T7（PSS、功能和步态的p < 0.01；COI的p = 0.01；PIS的p < 0.05）观察到显著差异。
自体血小板疗法在受试对象中使用未发现明显危害。单次给药产生了显著改善，且持续数月，因此值得进一步研究。
