Chateau G, Champalbert J
Rev Fr Transfus Immunohematol. 1978 Mar;21(2):533-44. doi: 10.1016/s0338-4535(78)80092-0.
Increasing of systematic syphilis screening has led many scientists to think to automatisation of classical cardiolipid reactions. The Debains-Kolmer reactions have been adapted to continuous flow but they appear actually inefficient. The use of ART (automated reagin test) gives very satisfying results and this reagent has obtained its homologation in the United States. The apparition of Groupamatic has incitated M. GARRETTA to adapt on this material the K. Antigen reagent manufactured by the Blood Transfusion Center in Lille and has led to the definition of GAST reagent (Groupamatic automated syphilis test). The authors describe the method of preparation of this reagent and its utilization on Groupamatic. It appears that the bovin cardiolipidic extract, non specific by definition, lays down to antagonist problems: to be sensitive enough when specific enough. It's the final reagent settlement which is the more delicate and which needs the maximum in manipulation. Yet, the composition of GAST is now well established and this reagent, ready for use, may be kept during three months at 4 degrees C. Then the authors describe the results of their own use of GAST, in routine on Groupamatic 360: out of 6 079 plasmas of blood donnors examined, 3 per cent of reactions are positive or doubtfull (which stays compatible with the screenings in large number). After confirmation with complementary tests made with manually GAST, RPR with microscopic reading, haemagglutination, and lastly fluorescent method, it appears that the rate of positive reactions is 1,7%. This result is conformable to habitual statistics. In conclusion, the GAST is a well-adapted reagent for Groupamatic technology, and represents a progress compared with classical manually methods. The adaptation of syphilis screening on Groupamatic is a factor of rentability of this equipment, allowing to realize 360 tests in one hour. At last, considering that cardiolipidic reactions are not of an absolute diagnostic value, treponemic complementary tests are necessary in order to confirm positive results picked up on Groupamatic.
系统性梅毒筛查的增加促使许多科学家思考经典心磷脂反应的自动化。德班 - 科尔默反应已被应用于连续流动，但实际上效率不高。使用自动反应素试验（ART）能得到非常令人满意的结果，并且该试剂在美国已获得认证。Groupamatic的出现促使M. 加雷塔将里尔输血中心生产的K抗原试剂应用于该设备，并由此定义了GAST试剂（Groupamatic自动梅毒检测）。作者描述了该试剂的制备方法及其在Groupamatic上的使用。看来，从定义上讲不具有特异性的牛心磷脂提取物存在拮抗问题：特异性足够时要足够灵敏。这是最终试剂的调配，是最精细的部分，需要极其小心地操作。然而，GAST的成分现已确定，这种即用型试剂在4摄氏度下可保存三个月。然后作者描述了他们在Groupamatic 360上常规使用GAST的结果：在检测的6079份献血者血浆中，3%的反应呈阳性或可疑（这与大量筛查结果相符）。在用手动GAST、显微镜读数的快速血浆反应素环状卡片试验（RPR）、血凝试验以及最后荧光法进行补充检测确认后，阳性反应率为1.7%。这一结果与常规统计数据相符。总之，GAST是一种非常适合Groupamatic技术的试剂，与传统手工方法相比有进步。将梅毒筛查应用于Groupamatic是该设备提高收益的一个因素，一小时可完成360次检测。最后，鉴于心磷脂反应并非具有绝对诊断价值，为了确认在Groupamatic上检测到的阳性结果，进行梅毒螺旋体补充检测是必要的。
