Duan He, Qiu Jian
Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan East Road in Huanggu District, Shenyang, 110036, Liaoning, China.
Discov Oncol. 2022 Apr 9;13(1):23. doi: 10.1007/s12672-022-00485-2.
CircRNAs are functional in cancer-related processes and are promising candidates for cancer prognostic biomarkers. The study aimed to evaluate the functional and clinical significance of has_circ_0001944 in colorectal cancer (CRC), including predictive value for overall survival (OS) and recurrence-free survival (RFS), and its effect on cell growth and metastasis.
This retrospective study included 133 patients with CRC. The expression of has_circ_0001944 in tissues and cells was quantified by real-time quantitative reverse transcription PCR. Receiver operating characteristics and Kaplan-Meier survival analysis were used to assess the significance of has_circ_0001944 as a prognostic marker, and its reliability was validated using multivariate regression analysis. Subsequently, XTT, transwell migration, and modified-transwell invasion assays were used to determine the behavior of the CRC cells in response to has_circ_0001944 inhibition.
Results of the qRT-PCR showed upregulation of has_circ_0001944 in the CRC samples compared to the normal samples. High has_circ_0001944 expression indicated shorter OS and RFS, comes down to poor prognosis. Multivariate regression analysis showed that elevated has_circ_0001944 increased the risk of death or recurrence and is a valuable prognostic factor. Following the has_circ_0001944 inhibition, the proliferation, migration and invasion of the CRC cells were reduced. miR-548b-3p was target miRNA of has_circ_0001944.
Up-regulation of has_circ_0001944 is associated with a poor prognosis of CRC. has_circ_0001944 downregulation can slow the progression of CRC partly by targeting miR-548b-3p.
环状RNA在癌症相关过程中发挥作用，是癌症预后生物标志物的有潜力候选者。本研究旨在评估has_circ_0001944在结直肠癌（CRC）中的功能和临床意义，包括对总生存期（OS）和无复发生存期（RFS）的预测价值，以及其对细胞生长和转移的影响。
这项回顾性研究纳入了133例CRC患者。通过实时定量逆转录PCR定量检测组织和细胞中has_circ_0001944的表达。采用受试者工作特征曲线和Kaplan-Meier生存分析评估has_circ_0001944作为预后标志物的意义，并通过多变量回归分析验证其可靠性。随后，使用XTT、Transwell迁移和改良Transwell侵袭试验来确定CRC细胞对has_circ_0001944抑制的反应行为。
qRT-PCR结果显示，与正常样本相比，CRC样本中has_circ_0001944表达上调。has_circ_0001944高表达表明OS和RFS较短，预后较差。多变量回归分析显示，has_circ_0001944升高增加了死亡或复发风险，是一个有价值的预后因素。抑制has_circ_0001944后，CRC细胞的增殖、迁移和侵袭减少。miR-548b-3p是has_circ_0001944的靶标miRNA。
has_circ_0001944上调与CRC预后不良相关。has_circ_0001944下调可部分通过靶向miR-548b-3p减缓CRC进展。
