阿片肽在脊髓刺激治疗神经病理性疼痛大鼠模型中的镇痛作用。
Involvement of Opioid Peptides in the Analgesic Effect of Spinal Cord Stimulation in a Rat Model of Neuropathic Pain.
机构信息
Neuroscience Research Institute, Peking University, Beijing, 100191, China.
Department of Neurobiology, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.
出版信息
Neurosci Bull. 2022 Apr;38(4):403-416. doi: 10.1007/s12264-022-00844-7. Epub 2022 Apr 9.
Abstract
Spinal cord stimulation (SCS)-induced analgesia was characterized, and its underlying mechanisms were examined in a spared nerve injury model of neuropathic pain in rats. The analgesic effect of SCS with moderate mechanical hypersensitivity was increased with increasing stimulation intensity between the 20% and 80% motor thresholds. Various frequencies (2, 15, 50, 100, 10000 Hz, and 2/100 Hz dense-dispersed) of SCS were similarly effective. SCS-induced analgesia was maintained without tolerance within 24 h of continuous stimulation. SCS at 2 Hz significantly increased methionine enkephalin content in the cerebrospinal fluid. The analgesic effect of 2 Hz was abolished by μ or κ opioid receptor antagonist. The effect of 100 Hz was prevented by a κ antagonist, and that of 10 kHz was blocked by any of the μ, δ, or κ receptor antagonists, suggesting that the analgesic effect of SCS at different frequencies is mediated by different endorphins and opioid receptors.
摘要
脊髓刺激 (SCS) 诱导的镇痛作用在大鼠神经病理性疼痛的 spared 神经损伤模型中得到了表征，并研究了其潜在机制。在具有中度机械性超敏反应的情况下，SCS 的镇痛作用随着刺激强度从 20%增加到 80%运动阈值而增加。各种频率（2、15、50、100、10000 Hz 和 2/100 Hz 密集分散）的 SCS 同样有效。在连续刺激 24 小时内，SCS 诱导的镇痛作用没有产生耐受。2 Hz 的 SCS 显著增加了脑脊液中的甲硫氨酸脑啡肽含量。2 Hz 的镇痛作用被 μ 或 κ 阿片受体拮抗剂所阻断。100 Hz 的作用被 κ 拮抗剂所预防，而 10 kHz 的作用则被任何 μ、δ 或 κ 受体拮抗剂所阻断，这表明不同频率的 SCS 的镇痛作用是由不同的内啡肽和阿片受体介导的。
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