Division of Rheumatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
Division of Rheumatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
Clin Immunol. 2022 May;238:108999. doi: 10.1016/j.clim.2022.108999. Epub 2022 Apr 7.
Many variants of SARS-CoV-2 have emerged, and decreased neutralizing antibodies after vaccination and breakthrough infections have become a problem. The importance of monitoring titers of neutralizing antibodies is getting higher. We enrolled 146 COVID-19 patients, who were thought to be infected with Wuhan-hu-1 or D614G strains, and examined the time course of neutralizing titers against six concerning strains (Wuhan-hu-1, Alpha, Beta, Gamma, Kappa, and Delta) using newly developed ELISA. The acquisition of neutralizing titer was positively associated with disease severity. Immune evasions were observed approximately 20 to 30% for Alpha, Kappa, and Delta variant, and 40 to 45% for Beta and Gamma variant. The titers against all strains decreased over time, and interestingly, while titers against Wuhan-hu-1 decreased by 23%, those to Delta variant decreased by 70%. Our simple, cost-effective, and non-hazardous system will be applicable to process numerous samples, such as monitoring titers against prevalent strains after infection or vaccination.
已经出现了许多 SARS-CoV-2 的变体，疫苗接种和突破感染后中和抗体滴度下降已成为一个问题。监测中和抗体滴度的重要性越来越高。我们招募了 146 名 COVID-19 患者，他们被认为感染了武汉株或 D614G 株，并使用新开发的 ELISA 检测了针对六种关注株（武汉株、Alpha、Beta、Gamma、Kappa 和 Delta）的中和抗体滴度的时间过程。中和抗体滴度的获得与疾病严重程度呈正相关。大约观察到 Alpha、Kappa 和 Delta 变体有 20%到 30%的免疫逃逸，而 Beta 和 Gamma 变体有 40%到 45%的免疫逃逸。所有株的抗体滴度随时间下降，有趣的是，武汉株的抗体滴度下降了 23%，而 Delta 变体的抗体滴度下降了 70%。我们的简单、经济实惠且无危险的系统将适用于处理大量样本，例如在感染或接种疫苗后监测针对流行株的抗体滴度。
