采用固相萃取/气相色谱-质谱联用技术同时分析米糠中的游离植物甾醇和植物甾醇糖苷。

Simultaneous analysis of free phytosterols and phytosterol glycosides in rice bran by SPE/GC-MS.

作者信息

Feng Simin, Wang Liling, Shao Ping, Lu Baiyi, Chen Yanfei, Sun Peilong

机构信息

Department of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, People's Republic of China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, People's Republic of China.

Department of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, People's Republic of China.

出版信息

Food Chem. 2022 Sep 1;387:132742. doi: 10.1016/j.foodchem.2022.132742. Epub 2022 Mar 19.

DOI:10.1016/j.foodchem.2022.132742

PMID:35398677

Abstract

In this study, free phytosterols and phytosterol glycosides in rice bran were successfully separated and analyzed using solid-phase extraction (SPE) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that free phytosterols in rice bran included ergosterol (129 ± 8 μg/g rice bran), campesterol (126 ± 9 μg/g rice bran), stigmasterol (106 ± 9 μg/g rice bran), β-sitosterol (305 ± 10 μg/g rice bran), cycloartenol (80.5 ± 3.9 μg/g rice bran) and 24-methylenecycloartenol (87.1 ± 2.2 μg/g rice bran), while phytosterol glycosides included campesterol glucoside (16.0 ± 1.3 μg/g rice bran), stigmasterol glucoside (99.0 ± 4.9 μg/g rice bran) and β-sitosterol glucoside (133 ± 7 μg/g rice bran). The methodological validation indicated that this method could accurately quantify free phytosterols and phytosterol glycosides in rice bran. This study provided a new direction to establish a rapid and simple method for the simultaneous determination of different forms of phytosterols in foods.

摘要

在本研究中，采用固相萃取（SPE）结合气相色谱-质谱联用（GC-MS）技术成功分离并分析了米糠中的游离植物甾醇和植物甾醇糖苷。结果表明，米糠中的游离植物甾醇包括麦角甾醇（129±8μg/g米糠）、菜油甾醇（126±9μg/g米糠）、豆甾醇（106±9μg/g米糠）、β-谷甾醇（305±10μg/g米糠）、环阿屯醇（80.5±3.9μg/g米糠）和24-亚甲基环阿屯醇（87.1±2.2μg/g米糠），而植物甾醇糖苷包括菜油甾醇葡萄糖苷（16.0±1.3μg/g米糠）、豆甾醇葡萄糖苷（99.0±4.9μg/g米糠）和β-谷甾醇葡萄糖苷（133±7μg/g米糠）。方法学验证表明，该方法能够准确测定米糠中游离植物甾醇和植物甾醇糖苷的含量。本研究为建立一种快速简便的同时测定食品中不同形式植物甾醇的方法提供了新方向。