Dong Yuhui, Ou Xichao, Liu Chunfa, Fan Weixing, Zhao Yanlin, Zhou Xiangmei
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People's Republic of China.
National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People's Republic of China.
Infect Drug Resist. 2022 Apr 2;15:1467-1475. doi: 10.2147/IDR.S346724. eCollection 2022.
Glycerol kinase () is essential for the first step of glycerol catabolism in . However, has been known to grow poorly in glycerol media because of a base insertion in the gene.
We analyzed the gene sequences of 60 clinical isolates, and determined the minimum inhibitory concentration of 14 drugs by microdilution method to evaluate the effect of frameshift mutations on drug sensitivity. The effect of growth rate on its drug sensitivity was investigated using bacteria grown on glycerol or pyruvate.
A total of 44 (73.33%) clinical isolates have frameshift mutations in a homopolymeric tract of 7 cytosines in the gene. 15.00% isolates showed phenotypic drug resistance. Glycerol metabolism-deficient showed reduced susceptibility to 9 out of 14 tested drugs. Mutations in the gene can lead to impaired growth in glycerol-based media, while the minimal inhibitory concentration values of slow-growing were higher.
Mutations in the gene can lead to slowed growth and reduced susceptibility to drugs in , which may contribute to the emergence of drug-resistant and pose a threat to human health owing to the zoonotic capacity of .
甘油激酶（）对于甘油在中的分解代谢第一步至关重要。然而，已知由于基因中的碱基插入，在甘油培养基中生长较差。
我们分析了60株临床分离株的基因序列，并通过微量稀释法测定了14种药物的最低抑菌浓度，以评估移码突变对药物敏感性的影响。使用在甘油或丙酮酸上生长的细菌研究生长速率对其药物敏感性的影响。
共有44株（73.33%）临床分离株在基因中7个胞嘧啶的同聚物区域存在移码突变。15.00%的分离株表现出表型耐药。甘油代谢缺陷型对14种测试药物中的9种敏感性降低。基因中的突变可导致在基于甘油的培养基中生长受损，而生长缓慢的最低抑菌浓度值更高。
基因中的突变可导致生长减慢和对药物的敏感性降低，这可能有助于耐药菌的出现，并因其人畜共患病能力对人类健康构成威胁。
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