Wu Yinling, Zhang Miao, Zhao Qinglei, Chen Lanyu, Wang Guifang, Ge Shaoqin, Wang Shusong
Hebei University Health Science Center, Hebei University, Baoding, China.
NHC Key Laboratory of Family Planning and Healthy, Hebei Key Laboratory of Reproductive Medicine, Hebei Research Institute for Family Planning Science and Technology, Shijiazhuang, China.
Transl Androl Urol. 2022 Mar;11(3):336-347. doi: 10.21037/tau-22-115.
At present, the incidence of obesity is increasing. Several studies have shown that obesity can reduce male fertility by affecting spermatogenesis and semen quality. Traditional Chinese medicine (TCM) has fewer side effects and stable efficacy in the treatment of related diseases. This study aimed to investigate the effect of Huaji Jianpi Decoction on the semen quality of high-fat diet-induced obese mice.
The obese male mice model was constructed by using high-fat diet and the Huaji Jianpi Decoction was processed into an aqueous extract. Mice were allocated in the normal group (n=30) and five different treatment groups (n=50). Huaji Jianpi Decoction was applied in low-, medium- and high-dose [17.52 g/(kg·d), 35.04 g/(kg·d) and 70.07 g/(kg·d), respectively]. The body weight, body fat, testis wet weight, testis coefficient, and routine sperm parameters were detected and analyzed. Meanwhile, transmission electron microscope (TEM) was used to observe testis ultrastructure. reverse-transcription quantitative PCR (RT-qPCR) was used to measure the expression of tumour necrosis factor α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1).
Compared with normal mice (ND), the testis wet weight and testis coefficient of mice in the blank group were significantly decreased, while the number of mitochondria was observed to be decreased on testis ultrastructure examination, and apoptotic cells and germ cells in the spermatogenic tubules were shed. After Huaji Jianpi Decoction administration, the body fat and blood lipid levels of obese mice were decreased, the testis wet weight and testis coefficient were increased, and semen parameters were increased. Different doses of Huaji Jianpi Decoction could improve testicular weight, sperm density, sperm motility, forward motility, and total sperm motility. Huaji Jianpi Decoction could also downregulate TNF-α and MCP-1 expression and inhibit germ cell apoptosis to improve semen quality.
Huaji Jianpi Decoction can improve the semen quality of high-fat diet-induced obese mice by reducing weight and lipid levels.
目前，肥胖症的发病率正在上升。多项研究表明，肥胖可通过影响精子发生和精液质量降低男性生育能力。中药在相关疾病的治疗中副作用较少且疗效稳定。本研究旨在探讨化积健脾汤对高脂饮食诱导的肥胖小鼠精液质量的影响。
采用高脂饮食构建肥胖雄性小鼠模型，并将化积健脾汤制成水提取物。将小鼠分为正常组（n = 30）和五个不同治疗组（n = 50）。分别以低、中、高剂量[分别为17.52 g/(kg·d)、35.04 g/(kg·d)和70.07 g/(kg·d)]给予化积健脾汤。检测并分析体重、体脂、睾丸湿重、睾丸系数和常规精子参数。同时，使用透射电子显微镜（TEM）观察睾丸超微结构。采用逆转录定量聚合酶链反应（RT-qPCR）检测肿瘤坏死因子α（TNF-α）和单核细胞趋化蛋白-1（MCP-1）的表达。
与正常小鼠（ND）相比，空白组小鼠的睾丸湿重和睾丸系数显著降低，睾丸超微结构检查观察到线粒体数量减少，生精小管中的凋亡细胞和生殖细胞脱落。给予化积健脾汤后，肥胖小鼠的体脂和血脂水平降低，睾丸湿重和睾丸系数增加，精液参数提高。不同剂量的化积健脾汤均可改善睾丸重量、精子密度、精子活力、前向运动能力和总精子活力。化积健脾汤还可下调TNF-α和MCP-1表达并抑制生殖细胞凋亡以改善精液质量。
化积健脾汤可通过减轻体重和降低血脂水平改善高脂饮食诱导的肥胖小鼠的精液质量。
