Alkhuriji Afrah F, Majrashi Nada A, Alomar Suliman, El-Khadragy Manal F, Awad Manal A, Khatab Alaa R, Yehia Hany M
Department of Zoology, College of Science, King Saud University, Riyadh 11495, Saudi Arabia.
Doping Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh 11495, Saudi Arabia.
Animals (Basel). 2020 Mar 29;10(4):573. doi: 10.3390/ani10040573.
is a psychrophilic bacterium, which causes widespread zoonosis in the natural environment, and mainly affects goat, sheep, and cattle herds. Recently, we predicted that it can be transmitted through food. It causes listeriosis, a severe infectious disease, which occurs with food contaminated with the pathogenic bacterium. Anti-inflammatory factors are important to treat the dangers of chronic inflammation associated with chronic diseases. Natural foodstuffs have made and are continuing to make vital contributions to the search for new antilisterial agents. The use of natural products in association with silver nanoparticles has drawn attention because of its easy, nonpathogenic, eco-friendly, and economical protocol. Hence, we aimed to biosynthesize silver nanoparticles (Ag-NPs) using peel extract, which was found to be a good source for the synthesis of silver nanoparticles, their formation being confirmed by color change and stability in solution, and investigated the antilisterial activity of these nanoparticles in a murine model of infection. A total of 28 mice were divided into four groups-healthy control, infected, infected mice treated with green Ag-NPs biosynthesized with (5 mg/mL), and infected mice pretreated with Ag-NPs. From our results, oral treatment with Ag-NPs biosynthesized with peel extract resulted in a significant reduction in malondialdehyde (MDA), enhanced antioxidant enzyme activities, and increased the levels of the antiapoptotic protein, compared with the untreated mice. These results indicate that may provide therapeutic value against -induced oxidative stress and histopathological alterations, and that these effects may be related to antiapoptotic and antioxidant activities.
是一种嗜冷细菌，在自然环境中引起广泛的人畜共患病，主要影响山羊、绵羊和牛群。最近，我们预测它可以通过食物传播。它会导致李斯特菌病，一种严重的传染病，与被这种病原菌污染的食物有关。抗炎因子对于治疗与慢性疾病相关的慢性炎症的危险很重要。天然食品已经并将继续为寻找新的抗李斯特菌剂做出重要贡献。天然产物与银纳米颗粒联合使用因其简便、无致病性、环保和经济的方案而受到关注。因此，我们旨在使用果皮提取物生物合成银纳米颗粒（Ag-NPs），发现其是合成银纳米颗粒的良好来源，通过颜色变化和溶液稳定性确认其形成，并在小鼠感染模型中研究这些纳米颗粒的抗李斯特菌活性。总共28只小鼠分为四组——健康对照、感染组、用（5mg/mL）生物合成的绿色Ag-NPs处理的感染小鼠组以及用Ag-NPs预处理的感染小鼠组。从我们的结果来看，与未处理的小鼠相比，用果皮提取物生物合成的Ag-NPs进行口服治疗导致丙二醛（MDA）显著降低、抗氧化酶活性增强以及抗凋亡蛋白水平升高。这些结果表明，可能对诱导的氧化应激和组织病理学改变具有治疗价值，并且这些作用可能与抗凋亡和抗氧化活性有关。
