Department of Ultrasound, The Third Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P. R. China.
Chongqing Key Laboratory of Ultrasound Molecular Imaging, Institute of Ultrasound Imaging, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P. R. China.
J Mater Chem B. 2022 May 11;10(18):3462-3473. doi: 10.1039/d1tb02308j.
Sequential nano-catalytic therapy has emerged as a novel therapeutic modality for cancer treatment as it utilizes the unique tumor microenvironment for selective tumor treatment. This study reports a magnetic nanoparticle to achieve Fenton-like reaction and dual-imaging guidance/monitoring. Natural glucose oxidase (GOx) and superparamagnetic FeO nanoparticles have been integrated into poly(lactic--glycolic acid) (PLGA) to fabricate a sequential nanocatalyst (designated as GOx@PLGA-FeO). This nanocatalyst can functionally deplete glucose in tumor tissues, producing a considerable amount of highly cytotoxic hydroxyl radicals the sequential Fenton-like reaction, and meanwhile maximizing the potential imaging capability as a contrast agent for magnetic resonance imaging and photoacoustic imaging. By ribonucleic acid sequencing (RNA-seq) technology, GOx@PLGA-FeO nanoparticles are demonstrated to induce tumor cell death by inhibiting multiple gene regulation pathways involving tumor growth and recurrence. Therefore, this finding provides a novel strategy to achieve promising therapeutic efficacy by the rational design of multifunctional nanoparticles with various features, including magnetic targeting, sequential nano-catalytic therapy, and dual-imaging guidance/monitoring.
序贯纳米催化治疗作为一种新型的癌症治疗方法已经出现，因为它利用了独特的肿瘤微环境进行选择性肿瘤治疗。本研究报告了一种利用磁性纳米颗粒实现类芬顿反应和双成像指导/监测的方法。天然葡萄糖氧化酶（GOx）和超顺磁性 FeO 纳米颗粒已被整合到聚（乳酸-乙醇酸）（PLGA）中，以制备序贯纳米催化剂（命名为 GOx@PLGA-FeO）。这种纳米催化剂可以在肿瘤组织中有效地消耗葡萄糖，产生大量的高细胞毒性羟基自由基，从而实现序贯类芬顿反应，并最大限度地发挥作为磁共振成像和光声成像对比剂的潜在成像能力。通过核糖核酸测序（RNA-seq）技术，GOx@PLGA-FeO 纳米颗粒通过抑制涉及肿瘤生长和复发的多种基因调控途径来诱导肿瘤细胞死亡。因此，这一发现为通过合理设计具有多种特性的多功能纳米颗粒（包括磁性靶向、序贯纳米催化治疗和双成像指导/监测）实现有前途的治疗效果提供了一种新策略。
