National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University.
Laboratory Department, Fifth affiliated (Zhuhai) Hospital of Zunyi Medical University.
J Vis Exp. 2022 Mar 22(181). doi: 10.3791/63364.
White blood cell (WBC) is an important indicator of inflammation in the body, and it can help distinguish between bacterial and viral infections. At present, most primary medical institutions in China have a poor percentage of adoption of blood-testing technology, and a hematology detection system with a high price to performance ratio and easy operation is urgently needed in primary healthcare centers. This paper introduces the principle and operation procedures of a point-of-care testing (POCT) card-based leukocyte analyzer (evaluated system), which was used to detect WBC indexes such as neutrophils, lymphocytes, and intermediate group cells (including eosinophils, basophils, and monocytes) in whole blood. The results from the evaluated system were compared to those from two commercial automatic hematology analyzers (reference system). The correlation and consistency between the evaluated system and the commercial reference systems were analyzed. The results showed that WBC count and number of granulocytes detected by the evaluated and reference systems showed a strong positive correlation (rs = 0.972 and 0.973, respectively), while the number of lymphocytes showed a relatively low correlation (rs = 0.851). A Bland-Altman plot showed that the major difference between the values detected by the evaluated system and the reference systems is within 95% limits of agreement (LoA), indicating that the two systems are in good agreement. In conclusion, the evaluated system has an excellent correlation, robust consistency, and a reliable comparison with the results of the widely used automatic hematology analyzers. It is ideal for WBC detection in primary medical institutions where a full-automatic five-category hematology analyzer is unavailable, especially during the COVID-19 normalized prevention and control period.
白细胞（WBC）是体内炎症的一个重要指标，它可以帮助区分细菌感染和病毒感染。目前，中国大多数基层医疗机构对血液检测技术的采用率较低，基层医疗卫生机构急需一种性价比高、操作简单的血液检测系统。本文介绍了一种即时检测（POCT）卡式白细胞分析仪（评价系统）的原理和操作步骤，该系统用于检测全血中的中性粒细胞、淋巴细胞和中间群细胞（包括嗜酸性粒细胞、嗜碱性粒细胞和单核细胞）等 WBC 指标。将评价系统的结果与两种商用全自动血液分析仪（参考系统）的结果进行比较。分析评价系统与商用参考系统之间的相关性和一致性。结果表明，评价系统和参考系统检测的 WBC 计数和粒细胞计数呈强正相关（rs 值分别为 0.972 和 0.973），而淋巴细胞计数的相关性相对较低（rs 值为 0.851）。Bland-Altman 图显示，评价系统和参考系统检测值之间的主要差异在 95%一致性界限（LoA）内，表明两种系统具有良好的一致性。总之，评价系统与广泛应用的全自动血液分析仪结果具有极好的相关性、稳健的一致性，可用于无法使用全自动五分类血液分析仪的基层医疗机构，特别是在 COVID-19 常态化防控期间。
