Department of Medical Support Technology, Institute of Systematic Engineering, Academy of Military Science, Tianjin, 300161, PR China.
National Bio-Protection Engineering Center, Tianjin, 300161, PR China.
Lab Chip. 2022 May 3;22(9):1702-1713. doi: 10.1039/d1lc01056e.
Traditional detection methods have shortcomings such as time-consumption and requirement of large instruments, which cannot meet the demands for on-site detection or analysis. Silicon nanowire-field-effect transistor (SiNW-FET) biosensors have the advantages of high speed, high sensitivity, strong specificity, and ease of integration. However, SiNW-FET biosensors also have some demerits: they are too sensitive, environmental factors such as light, temperature, and pH easily cause interference, and their performance uniformity needs to be calibrated in advance. In this work, we constructed a self-contained and integrated microfluidic nano-detection system containing a SiNW-FET biosensor for bio-detection and analysis. All analysis processes including liquid sample delivery, optical modulation, constant temperature control, signal amplification and data acquisition, and result display were automatically performed. In series tests including light-guided ones by analyzing various types of samples with an automatic sample injection mode, the system shows good stability and robustness. Its signal accuracy was verified using a commercial high-precision ammeter ( = 0.9988), too. The feasibility of the system for bio-detection was verified using simulant samples of the typical microorganism with a limit of detection of 1.0 fg mL. Furthermore, the process of the binding-dissociation of antibody-protein pairs was analyzed using the system, demonstrating the potential for molecular interaction analysis. This system is highly integrated, small in size, and easy to carry, which will be developed into a portable device for on-site bio-detection and analysis of molecular interactions to enable environmental testing, medical research, food and agricultural safety, military medicine,
传统的检测方法存在耗时和需要大型仪器等缺点，无法满足现场检测或分析的需求。硅纳米线场效应晶体管（SiNW-FET）生物传感器具有高速、高灵敏度、强特异性和易于集成的优点。然而，SiNW-FET 生物传感器也存在一些缺点：它们过于敏感，光、温度和 pH 等环境因素容易引起干扰，并且其性能均匀性需要预先校准。在这项工作中，我们构建了一个包含 SiNW-FET 生物传感器的自包含式集成微流控纳米检测系统，用于生物检测和分析。所有分析过程，包括液体样品输送、光学调制、恒温控制、信号放大和数据采集以及结果显示，都自动执行。在包括通过自动进样模式分析各种类型的样品进行的光引导测试在内的一系列测试中，该系统显示出良好的稳定性和鲁棒性。它的信号准确性也通过使用商业高精度安培计（=0.9988）进行了验证。使用典型微生物的模拟样品进行生物检测的可行性也通过该系统得到了验证，检测限为 1.0 fg mL。此外，该系统还分析了抗体-蛋白质结合物的结合-解离过程，展示了用于分子相互作用分析的潜力。该系统高度集成，体积小，易于携带，将被开发成一种用于现场生物检测和分析分子相互作用的便携式设备，以实现环境测试、医学研究、食品和农业安全、军事医学等领域的应用。
