Department of Emergency Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Department of Emergency Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Toxicology. 2022 Apr 30;472:153178. doi: 10.1016/j.tox.2022.153178. Epub 2022 Apr 9.
In recent decades, Bisphenol S (BPS), which was once thought to be an alternative for Bisphenol A (BPA) has been extensively used in personal care products, paper products, and food. However, there is an unclear association between bisphenol and tumors. Therefore, clarifying this relationship is critical for disease prevention and treatment. This work found a novel method that predicts a correlation between bisphenol interactive genes and tumors. First, the transcriptome profile and interactive genes of bisphenol were obtained from The Cancer Genome Atlas and Genotype-Tissue Expression, Comparative Toxicology Genomics, and PharmMapper databases. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that interactive genes are primarily enriched in prostate cancer. Gene targeted prediction and gene set variation analysis confirmed that bisphenol exerts potential effects on prostate cancer. The operating characteristic curves and survival analysis uncovered the role of COL1A1 and COL1A2 in predicting the prognosis of prostate cancer. Cell counting kit-8 assay revealed that BPS-treated cells could remarkably promote cell proliferation capacity in both PC-3 and LNCap cells. In addition, wound healing and transwell assays demonstrated that BPS-treated cells could significantly promote the cell invasion capacity of prostate cells. Notably, two key genes, i.e., COL1A1 and COL1A2 were significantly upregulated with BPS-treated PC-3 and LNCap cells.
在最近几十年中，双酚 S（BPS）曾被认为是双酚 A（BPA）的替代品，被广泛应用于个人护理产品、纸制品和食品中。然而，双酚与肿瘤之间的关联尚不清楚。因此，澄清这种关系对于疾病的预防和治疗至关重要。这项工作发现了一种预测双酚相互作用基因与肿瘤之间相关性的新方法。首先，从癌症基因组图谱和基因型组织表达、比较毒理学基因组学和 PharmMapper 数据库中获得了双酚的转录组谱和相互作用基因。基因本体论和京都基因与基因组百科全书分析表明，相互作用基因主要富集在前列腺癌中。基因靶向预测和基因集变异分析证实了双酚对前列腺癌具有潜在影响。工作特征曲线和生存分析揭示了 COL1A1 和 COL1A2 在预测前列腺癌预后中的作用。细胞计数试剂盒-8 测定表明，BPS 处理的细胞可显著促进 PC-3 和 LNCap 细胞的增殖能力。此外，划痕愈合和 Transwell 测定表明，BPS 处理的细胞可显著促进前列腺细胞的侵袭能力。值得注意的是，COL1A1 和 COL1A2 这两个关键基因在 BPS 处理的 PC-3 和 LNCap 细胞中显著上调。
