bZIP 转录因子 PavbZIP6 通过增加甜樱桃中的脱落酸来调节花色苷的积累。

bZIP Transcription Factor PavbZIP6 Regulates Anthocyanin Accumulation by Increasing Abscisic Acid in Sweet Cherry.

机构信息

Frontiers Science Center for Molecular Design Breeding, College of Horticulture, China Agricultural University, Beijing 100193, China.

出版信息

Int J Mol Sci. 2024 Sep 23;25(18):10207. doi: 10.3390/ijms251810207.

DOI:10.3390/ijms251810207

PMID:39337692

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11432629/

Abstract

Basic leucine zipper (bZIP) transcription factors (TFs) play a crucial role in anthocyanin accumulation in plants. In addition to bZIP TFs, abscisic acid (ABA) increases anthocyanin biosynthesis. Therefore, this study aimed to investigate whether bZIP TFs are involved in ABA-induced anthocyanin accumulation in sweet cherry and elucidate the underlying molecular mechanisms. Specifically, the BLAST method was used to identify genes in sweet cherry. Additionally, we examined the expression of ABA- and anthocyanin-related genes in sweet cherry following the overexpression or knockdown of a candidate gene. In total, we identified 54 -encoding genes in the sweet cherry genome. () showed significantly increased expression, along with increased anthocyanin accumulation in sweet cherry. Additionally, yeast one-hybrid and dual-luciferase assays indicated that PavbZIP6 enhanced the expression of anthocyanin biosynthetic genes (, , and ), thereby increasing anthocyanin accumulation. Moreover, PavbZIP6 interacted directly with the promoter, thereby regulating to promote abscisic acid (ABA) synthesis and enhance anthocyanin accumulation in sweet cherry fruit. Conclusively, this study reveals a novel mechanism by which mediates anthocyanin biosynthesis in response to ABA and contributes to our understanding of the mechanism of genes in the regulation of anthocyanin biosynthesis in sweet cherry.

摘要

碱性亮氨酸拉链（bZIP）转录因子（TFs）在植物花色素苷积累中起着至关重要的作用。除了 bZIP TFs 外，脱落酸（ABA）也会增加花色素苷的生物合成。因此，本研究旨在探讨 bZIP TFs 是否参与了甜樱桃中 ABA 诱导的花色素苷积累，并阐明其潜在的分子机制。具体来说，使用 BLAST 方法鉴定甜樱桃中的基因。此外，我们还检测了在候选基因过表达或敲低后，甜樱桃中 ABA 和花色素苷相关基因的表达情况。总共鉴定出 54 个编码基因在甜樱桃基因组中。()表现出显著增加的表达，同时伴随着甜樱桃中花色素苷的积累增加。此外，酵母单杂交和双荧光素酶报告基因 assays 表明 PavbZIP6 增强了花色素苷生物合成基因（，，和）的表达，从而增加了花色素苷的积累。此外，PavbZIP6 与启动子直接相互作用，从而调节以促进脱落酸（ABA）的合成，并增强甜樱桃果实中花色素苷的积累。综上所述，本研究揭示了一种新的机制，即通过介导 ABA 响应下的花色素苷生物合成，参与了我们对甜樱桃中花色素苷生物合成调控机制的理解。