Jiang Shenghui, Chen Min, He Naibo, Chen Xiaoliu, Wang Nan, Sun Qingguo, Zhang Tianliang, Xu Haifeng, Fang Hongcheng, Wang Yicheng, Zhang Zongying, Wu Shujing, Chen Xuesen
1College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production in Shandong, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018 China.
National Oceanographic Center, 88 Xuzhou Road, Qingdao, 266071 China.
Hortic Res. 2019 Mar 1;6:40. doi: 10.1038/s41438-019-0118-6. eCollection 2019.
Anthocyanins are biosynthesized on the cytosolic surface of the endoplasmic reticulum and then transported into the vacuole for storage. Glutathione -transferases (GSTs) are considered to be responsible for the transport of anthocyanins into the vacuole. However, the regulatory mechanisms of GSTs in plants are still unclear. Here, we performed a genome-wide analysis and identified 69 genes in apple. The expression of was positively correlated with the anthocyanin content ( = 0.949) during 'Yanfu 8' fruit development. The overexpression of in the mutant resulted in seedlings of 35S::-GFP/ that could accumulate anthocyanin and rescue its phenotype, suggesting that MdGSTF6 was an anthocyanin transporter. The silencing of affected anthocyanin accumulation in apple fruit. Moreover, the knockdown of by RNA interference in cultured 'Gala' seedlings inhibited anthocyanin accumulation. The interaction experiments showed that MdMYB1 could bind directly to the promoter to transcriptionally activate its expression. Collectively, our results demonstrate that encodes an important GST transporter of anthocyanins in apple fruit and provide evidence for the associated regulatory mechanisms. Therefore, MdMYB1 can not only regulate anthocyanin synthesis, but also control the transport of anthocyanin in apples. This information may be useful for further clarifying the regulation of anthocyanin transport in apple.
花青素在内质网的胞质表面进行生物合成，然后被转运到液泡中储存。谷胱甘肽-S-转移酶（GSTs）被认为负责花青素向液泡的转运。然而，GSTs在植物中的调控机制仍不清楚。在此，我们进行了全基因组分析，并在苹果中鉴定出69个基因。在‘烟富8’果实发育过程中，其表达与花青素含量呈正相关（r = 0.949）。在突变体中过表达导致35S::-GFP/幼苗能够积累花青素并挽救其表型，表明MdGSTF6是一种花青素转运蛋白。MdGSTF6的沉默影响了苹果果实中花青素的积累。此外，在离体培养的‘嘎啦’幼苗中通过RNA干扰敲低MdGSTF6抑制了花青素的积累。相互作用实验表明，MdMYB1可以直接结合到MdGSTF6启动子上，转录激活其表达。总的来说，我们的结果表明MdGSTF6编码苹果果实中一种重要的花青素GST转运蛋白，并为相关调控机制提供了证据。因此，MdMYB1不仅可以调节花青素的合成，还可以控制苹果中花青素的转运。这些信息可能有助于进一步阐明苹果中花青素转运的调控机制。
