Yu Zhongyu, Tian Chang, Guan Yunxiao, He Jun, Wang Zhenxing, Wang Likai, Lin Sisi, Guan Zhiyong, Fang Weimin, Chen Sumei, Zhang Fei, Jiang Jiafu, Chen Fadi, Wang Haibin
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Key Laboratory of Biology of Ornamental Plants in East China, Ministry of Agriculture and Rural Affairs, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2022 Jun 2;13:860956. doi: 10.3389/fpls.2022.860956. eCollection 2022.
Autopolyploids often exhibit plant characteristics different from their diploid ancestors and are frequently associated with altered genes expression controlling growth and development. TCP is a unique transcription factor family in plants that is closely related to plant growth and development. Based on transcriptome sequencing of , 23 full-length genes were cloned. The expression of was most variable in tetraploids, at least threefold greater than diploids. Due to the lack of a transgenic system, we overexpressed in (Col-0) and . Overexpression of caused enlargement of leaves in and petals in , and the expression of genes downstream of the GA pathway in were increased. Our results suggest that autopolyploidization of led to differential expression of TCP family genes, thereby affecting plant characteristics by the GA pathway. This study improves the understanding of enlarged plant size after autopolyploidization.
同源多倍体通常表现出与其二倍体祖先不同的植物特征,并且经常与控制生长和发育的基因表达改变有关。TCP是植物中一个独特的转录因子家族,与植物生长和发育密切相关。基于转录组测序,克隆了23个全长基因。该基因的表达在四倍体中变化最大,至少比二倍体高3倍。由于缺乏该基因的转基因系统,我们在拟南芥(Col-0)和烟草中过表达了该基因。该基因的过表达导致拟南芥叶片增大、烟草花瓣增大,并且烟草中GA途径下游基因的表达增加。我们的结果表明,该植物的同源多倍体化导致TCP家族基因的差异表达,从而通过GA途径影响植物特征。本研究增进了对同源多倍体化后植物大小增大的理解。